| Title |
Exploring the enkephalinergic differentiation potential in adult stem cells for cell therapy and drug screening implications
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, October 2012
|
| DOI | 10.1007/s11626-012-9546-4 |
| Pubmed ID | |
| Authors |
Maryam Hafizi, Behnaz Bakhshandeh, Masoud Soleimani, Amir Atashi |
| Abstract |
Stem cell therapy is one of the most promising treatments in neuroregenerative medicine. Considering the role of the endogenous opioid system in controlling the pathophysiology of neurological disorders and behavioral aberrations, current studies have focused on enkephalins as a part of the opioid system. Due to high capability of unrestricted somatic stem cells (USSCs) and human mesenchymal stem cells (hMSCs) for cell therapy and transplantation; here, we examined their enkephalinergic differentiation potential through Ikaros-related pathways in order to develop in vitro models to help drug screening and stem cell therapy for the opioid-related disorders. The authenticity of the stem cells was verified by differentiation experiments along with flow cytometry for surface markers. Later, we confirmed their neurogenic differentiation with semiquantitative and quantitative transcriptional and translational evaluations of the enkephalinergic-related genes such as proenkephalin, CREBZF, Ikaros, and prodynorphin. Our findings supported the enkephalinergic differentiation of these stem cells. Noteworthy, USSCs showed higher potential for differentiating into enkephalinergic neurons under Ikaros activation than hMSCs, which makes them appropriate for neurological therapeutic applications. In conclusion, this study suggests a powerful in vitro model for neurogenesis that may help clarification of enkephalinergic differentiation and related signaling networks along with neural drug screening. Such investigations may be beneficial to ameliorate the neural-related therapeutic approaches. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 4 | 20% |
| Student > Bachelor | 4 | 20% |
| Student > Ph. D. Student | 2 | 10% |
| Other | 2 | 10% |
| Student > Master | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 4 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 20% |
| Biochemistry, Genetics and Molecular Biology | 3 | 15% |
| Medicine and Dentistry | 3 | 15% |
| Computer Science | 2 | 10% |
| Physics and Astronomy | 1 | 5% |
| Other | 3 | 15% |
| Unknown | 4 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2021.
All research outputs
#14,735,403
of 22,681,577 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#460
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Outputs of similar age
#104,621
of 172,672 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#3
of 4 outputs
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So far Altmetric has tracked 788 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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