| Title |
Sequential intravital imaging reveals in vivo dynamics of pancreatic tissue transplanted under the kidney capsule in mice
|
|---|---|
| Published in |
Diabetologia, July 2016
|
| DOI | 10.1007/s00125-016-4049-6 |
| Pubmed ID | |
| Authors |
Léon van Gurp, Cindy J. M. Loomans, Pim P. van Krieken, Gitanjali Dharmadhikari, Erik Jansen, Femke C. A. S. Ringnalda, Evelyne Beerling, Jacco van Rheenen, Eelco J. P. de Koning |
| Abstract |
Dynamic processes in pancreatic tissue are difficult to study. We aimed to develop an intravital imaging method to longitudinally examine engraftment, vascularisation, expansion and differentiation in mature islets or embryonic pancreases transplanted under the kidney capsule. Isolated pancreatic islets from adult mice and murine embryonic day (E)12.5 pancreases containing fluorescent biomarkers were transplanted under the kidney capsule of immunodeficient recipient mice. Human islet cells were dispersed, transduced with a lentivirus expressing a fluorescent label and reaggregated before transplantation. Graft-containing kidneys were positioned subcutaneously and an imaging window was fitted into the skin on top of the kidney. Intravital imaging using multiphoton microscopy was performed for up to 2 weeks. Volumes of fluorescently labelled cells were determined as a measure of development and survival. Transplanted islets and embryonic pancreases showed good engraftment and remained viable. Engraftment and vascularisation could be longitudinally examined in murine and human islet cells. Murine islet beta cell volume was unchanged over time. Transplanted embryonic pancreases increased to up to 6.1 times of their original volume and beta cell volume increased 90 times during 2 weeks. This method allows for repeated intravital imaging of grafts containing various sources of pancreatic tissue transplanted under the kidney capsule. Using fluorescent markers, dynamic information concerning engraftment or differentiation can be visualised and measured. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 20% |
| United States | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 5% |
| Unknown | 41 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 28% |
| Student > Ph. D. Student | 8 | 19% |
| Student > Bachelor | 4 | 9% |
| Student > Doctoral Student | 2 | 5% |
| Other | 2 | 5% |
| Other | 7 | 16% |
| Unknown | 8 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 15 | 35% |
| Medicine and Dentistry | 10 | 23% |
| Agricultural and Biological Sciences | 4 | 9% |
| Neuroscience | 2 | 5% |
| Immunology and Microbiology | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 10 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2016.
All research outputs
#14,616,637
of 25,732,188 outputs
Outputs from Diabetologia
#4,413
of 5,376 outputs
Outputs of similar age
#201,284
of 380,182 outputs
Outputs of similar age from Diabetologia
#53
of 81 outputs
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