Title |
Review of Beet pseudoyellows virus genome structure built the consensus genome organization of cucumber strains and highlighted the unique feature of strawberry strain
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Published in |
Virus Genes, July 2016
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DOI | 10.1007/s11262-016-1376-0 |
Pubmed ID | |
Authors |
Md. Shamim Akhter, Sachin Ashok Bhor, Ngcebo Hlalele, Masahiro Nao, Ken-Taro Sekine, Takashi Yaeno, Naoto Yamaoka, Masamichi Nishiguchi, Augustine Gubba, Kappei Kobayashi |
Abstract |
The complete nucleotide sequences of Beet pseudoyellows virus (BPYV)-MI (cucumber isolate; Matsuyama, Idai) genomic RNAs 1 and 2 were determined and compared with the previously sequenced Japanese cucumber strain (BPYV-JC) and a strawberry strain (BPYV-S). The RNA 2 of BPYV-MI showed 99 % nucleotide sequence identity with both BPYV-JC and -S having highly conserved eight ORFs. In contrast, the RNA1 of BPYV-MI showed sequence identities of 98 and 86 % with BPYV-JC and -S, respectively. Phylogenetic analysis of RNA-dependent RNA polymerase (RdRp) coding sequences from three fully sequenced BPYV strains and five partially sequenced cucurbit-infecting BPYV strains from Japan and South Africa has shown that cucurbit-infecting strains are closer to each other than to BPYV-S. In addition, the strawberry strain BPYV-S has an ORF2 in the downstream of RdRp gene in RNA1, but all the cucumber strains, BPYV-JC, -MI, and those from South Africa, lacked the ORF2 of RNA1, highlighting the difference between common BPYV cucumber strains and a unique strawberry strain. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 20% |
Student > Bachelor | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Student > Master | 1 | 10% |
Professor > Associate Professor | 1 | 10% |
Other | 0 | 0% |
Unknown | 4 | 40% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 30% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Social Sciences | 2 | 20% |
Unknown | 3 | 30% |