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X Demographics
Mendeley readers
Attention Score in Context
| Title |
CMRF-56+ blood dendritic cells loaded with mRNA induce effective antigen-specific cytotoxic T-lymphocyte responses
|
|---|---|
| Published in |
OncoImmunology, May 2016
|
| DOI | 10.1080/2162402x.2016.1168555 |
| Pubmed ID | |
| Authors |
Phillip D. Fromm, Michael S. Papadimitrious, Jennifer L. Hsu, Nicolas Van Kooten Losio, Nirupama D. Verma, Tsun Ho Lo, Pablo A. Silveira, Christian E. Bryant, Cameron J. Turtle, Rebecca L. Prue, Peter Vukovic, David J. Munster, Tomoko Nagasaki, Ross T. Barnard, Stephen M. Mahler, Sébastien A. Anguille, Zwi Berneman, Lisa G. Horvath, Kenneth F. Bradstock, Douglas E. Joshua, Georgina J. Clark, Derek N. J. Hart |
| Abstract |
There are numerous transcriptional, proteomic and functional differences between monocyte-derived dendritic cells (Mo-DC) and primary blood dendritic cells (BDC). The CMRF-56 monoclonal antibody (mAb) recognizes a cell surface marker, which is upregulated on BDC following overnight culture. Given its unique ability to select a heterogeneous population of BDC, we engineered a human chimeric (h)CMRF-56 IgG4 mAb to isolate primary BDC for potential therapeutic vaccination. The ability to select multiple primary BDC subsets from patients and load them with in vitro transcribed (IVT) mRNA encoding tumor antigen might circumvent the issues limiting the efficacy of Mo-DC. After optimizing and validating the purification of hCMRF-56(+) BDC, we showed that transfection of hCMRF-56(+) BDC with mRNA resulted in efficient mRNA translation and antigen presentation by myeloid BDC subsets, while preserving superior DC functions compared to Mo-DC. Immune selected and transfected hCMRF-56(+) BDC migrated very efficiently in vitro and as effectively as cytokine matured Mo-DC in vivo. Compared to Mo-DC, hCMRF-56(+) BDC transfected with influenza matrix protein M1 displayed superior MHC peptide presentation and generated potent antigen specific CD8(+) T-cell recall responses, while Wilms tumor 1 (WT1) transfected CMRF-56(+) BDC generated effective primary autologous cytotoxic T-cell responses. The ability of the combined DC subsets within hCMRF-56(+) BDC to present mRNA delivered tumor antigens merits phase I evaluation as a reproducible generic platform for the next generation of active DC immune therapies. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 22% |
| Student > Ph. D. Student | 5 | 19% |
| Professor | 2 | 7% |
| Other | 2 | 7% |
| Student > Master | 2 | 7% |
| Other | 4 | 15% |
| Unknown | 6 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 5 | 19% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Medicine and Dentistry | 4 | 15% |
| Neuroscience | 2 | 7% |
| Psychology | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 7 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 July 2016.
All research outputs
#15,518,326
of 25,374,917 outputs
Outputs from OncoImmunology
#1,158
of 2,178 outputs
Outputs of similar age
#163,041
of 312,438 outputs
Outputs of similar age from OncoImmunology
#23
of 64 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,178 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,438 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.