| Title |
A novel arrangement of zinc finger nuclease system for in vivo targeted genome engineering: the tomato LEC1-LIKE4 gene case
|
|---|---|
| Published in |
Plant Cell Reports, July 2016
|
| DOI | 10.1007/s00299-016-2031-x |
| Pubmed ID | |
| Authors |
Zoe Hilioti, Ioannis Ganopoulos, Sabna Ajith, Ioannis Bossis, Athanasios Tsaftaris |
| Abstract |
A selection-free, highly efficient targeted mutagenesis approach based on a novel ZFN monomer arrangement for genome engineering in tomato reveals plant trait modifications. How to achieve precise gene targeting in plants and especially in crops remains a long-sought goal for elucidating gene function and advancing molecular breeding. To address this issue, zinc finger nuclease (ZFN)-based technology was developed for the Solanum lycopersicum seed system. A ZFN architecture design with an intronic sequence between the two DNA recognition sites was evaluated for its efficiency in targeted gene mutagenesis. Custom engineered ZFNs for the developmental regulator LEAFY-COTYLEDON1-LIKE4 (L1L4) coding for the β subunit of nuclear factor Y, when transiently expressed in tomato seeds, cleaved the target site and stimulated imperfect repair driven by nonhomologous end-joining, thus, introducing mutations into the endogenous target site. The successful in planta application of the ZFN platform resulted in L1L4 mutations which conferred heterochronic phenotypes during development. Our results revealed that sequence changes upstream of the DNA binding domain of L1L4 can lead to phenotypic diversity including fruit organ. These results underscore the utility of engineered ZFN approach in targeted mutagenesis of tomato plant which may accelerate translational research and tomato breeding. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 13% |
| India | 1 | 13% |
| Greece | 1 | 13% |
| Unknown | 5 | 63% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 63% |
| Scientists | 3 | 38% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 1% |
| Greece | 1 | 1% |
| Zimbabwe | 1 | 1% |
| Unknown | 71 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 22% |
| Student > Ph. D. Student | 10 | 14% |
| Student > Master | 7 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Student > Bachelor | 4 | 5% |
| Other | 10 | 14% |
| Unknown | 23 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 36% |
| Biochemistry, Genetics and Molecular Biology | 14 | 19% |
| Unspecified | 1 | 1% |
| Chemical Engineering | 1 | 1% |
| Computer Science | 1 | 1% |
| Other | 3 | 4% |
| Unknown | 27 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 November 2019.
All research outputs
#7,063,261
of 23,576,969 outputs
Outputs from Plant Cell Reports
#697
of 2,233 outputs
Outputs of similar age
#117,379
of 367,432 outputs
Outputs of similar age from Plant Cell Reports
#11
of 41 outputs
Altmetric has tracked 23,576,969 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 2,233 research outputs from this source. They receive a mean Attention Score of 4.1. This one has gotten more attention than average, scoring higher than 68% of its peers.
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We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.