| Title |
Cell-free protein synthesis: the state of the art
|
|---|---|
| Published in |
Biotechnology Techniques, October 2012
|
| DOI | 10.1007/s10529-012-1075-4 |
| Pubmed ID | |
| Authors |
James W. Whittaker |
| Abstract |
Cell-free protein synthesis harnesses the synthetic power of biology, programming the ribosomal translational machinery of the cell to create macromolecular products. Like PCR, which uses cellular replication machinery to create a DNA amplifier, cell-free protein synthesis is emerging as a transformative technology with broad applications in protein engineering, biopharmaceutical development, and post-genomic research. By breaking free from the constraints of cell-based systems, it takes the next step towards synthetic biology. Recent advances in reconstituted cell-free protein synthesis (Protein synthesis Using Recombinant Elements expression systems) are creating new opportunities to tailor the reactions for specialized applications including in vitro protein evolution, printing protein microarrays, isotopic labeling, and incorporating nonnatural amino acids. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 232 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 2% |
| Germany | 3 | 1% |
| United Kingdom | 2 | <1% |
| Mexico | 1 | <1% |
| Colombia | 1 | <1% |
| Unknown | 220 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 50 | 22% |
| Researcher | 43 | 19% |
| Student > Bachelor | 41 | 18% |
| Student > Master | 31 | 13% |
| Other | 10 | 4% |
| Other | 27 | 12% |
| Unknown | 30 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 71 | 31% |
| Agricultural and Biological Sciences | 59 | 25% |
| Chemistry | 20 | 9% |
| Engineering | 19 | 8% |
| Chemical Engineering | 6 | 3% |
| Other | 25 | 11% |
| Unknown | 32 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2019.
All research outputs
#3,202,014
of 25,373,627 outputs
Outputs from Biotechnology Techniques
#79
of 2,762 outputs
Outputs of similar age
#23,075
of 194,150 outputs
Outputs of similar age from Biotechnology Techniques
#1
of 19 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,150 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.