| Title |
Harvest site influences the growth properties of adipose derived stem cells
|
|---|---|
| Published in |
Methods in Cell Science, October 2012
|
| DOI | 10.1007/s10616-012-9498-2 |
| Pubmed ID | |
| Authors |
Patricia E. Engels, Mathias Tremp, Paul J. Kingham, Pietro G. di Summa, René D. Largo, Dirk J. Schaefer, Daniel F. Kalbermatten |
| Abstract |
The therapeutic potential of adult stem cells may become a relevant option in clinical care in the future. In hand and plastic surgery, cell therapy might be used to enhance nerve regeneration and help surgeons and clinicians to repair debilitating nerve injuries. Adipose-derived stem cells (ASCs) are found in abundant quantities and can be harvested with a low morbidity. In order to define the optimal fat harvest location and detect any potential differences in ASC proliferation properties, we compared biopsies from different anatomical sites (inguinal, flank, pericardiac, omentum, neck) in Sprague-Dawley rats. ASCs were expanded from each biopsy and a proliferation assay using different mitogenic factors, basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) was performed. Our results show that when compared with the pericardiac region, cells isolated from the inguinal, flank, omental and neck regions grow significantly better in growth medium alone. bFGF significantly enhanced the growth rate of ASCs isolated from all regions except the omentum. PDGF had minimal effect on ASC proliferation rate but increases the growth of ASCs from the neck region. Analysis of all the data suggests that ASCs from the neck region may be the ideal stem cell sources for tissue engineering approaches for the regeneration of nervous tissue. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 18% |
| Researcher | 7 | 13% |
| Other | 6 | 11% |
| Student > Master | 5 | 9% |
| Student > Bachelor | 4 | 7% |
| Other | 12 | 22% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 21 | 38% |
| Agricultural and Biological Sciences | 10 | 18% |
| Biochemistry, Genetics and Molecular Biology | 6 | 11% |
| Veterinary Science and Veterinary Medicine | 2 | 4% |
| Neuroscience | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 12 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2012.
All research outputs
#20,656,161
of 25,373,627 outputs
Outputs from Methods in Cell Science
#857
of 1,026 outputs
Outputs of similar age
#158,340
of 202,171 outputs
Outputs of similar age from Methods in Cell Science
#5
of 7 outputs
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