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Measuring Survival of Adherent Cells with the Colony-Forming Assay

Overview of attention for article published in Cold Spring Harbor Protocols, August 2016
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Title
Measuring Survival of Adherent Cells with the Colony-Forming Assay
Published in
Cold Spring Harbor Protocols, August 2016
DOI 10.1101/pdb.prot087171
Pubmed ID
Authors

Lisa C. Crowley, Melinda E. Christensen, Nigel J. Waterhouse

Abstract

Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provide an indirect measurement of cell death. This can be achieved using the colony-forming assay described here. This protocol specifically applies to measurement of HeLa cells but can be used for most adherent cell lines with limited motility.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 165 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Hungary 1 <1%
Unknown 164 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 18%
Researcher 21 13%
Student > Bachelor 20 12%
Student > Master 18 11%
Student > Doctoral Student 10 6%
Other 24 15%
Unknown 43 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 61 37%
Agricultural and Biological Sciences 27 16%
Pharmacology, Toxicology and Pharmaceutical Science 8 5%
Medicine and Dentistry 7 4%
Immunology and Microbiology 4 2%
Other 13 8%
Unknown 45 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 April 2017.
All research outputs
#17,811,816
of 22,881,964 outputs
Outputs from Cold Spring Harbor Protocols
#570
of 971 outputs
Outputs of similar age
#268,581
of 366,376 outputs
Outputs of similar age from Cold Spring Harbor Protocols
#21
of 43 outputs
Altmetric has tracked 22,881,964 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 971 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 366,376 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.