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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Extracting DNA from ‘jaws’: high yield and quality from archived tiger shark (Galeocerdo cuvier) skeletal material
|
|---|---|
| Published in |
Molecular Ecology Resources, August 2016
|
| DOI | 10.1111/1755-0998.12580 |
| Pubmed ID | |
| Authors |
E. E. Nielsen, J. A. T. Morgan, S. L. Maher, J. Edson, M. Gauthier, J. Pepperell, B. J. Holmes, M. B. Bennett, J. R. Ovenden |
| Abstract |
Archived specimens are highly valuable sources of DNA for retrospective genetic/genomic analysis. However, often limited effort has been made to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here we assess and optimize the usefulness of abundant archived skeletal material from sharks as a source of DNA for temporal genomic studies. Six different methods for DNA extraction, encompassing two different commercial kits and three different protocols, were applied to material, so-called "bio-swarf", from contemporary and archived jaws and vertebrae of tiger sharks (Galeocerdo cuvier). Protocols were compared for DNA yield and quality using a qPCR approach. For jaw swarf all methods provided relatively high DNA yield and quality, while large differences in yield between protocols were observed for vertebrae. Similar results were obtained from samples of white shark (Carcharodon carcharias). Application of the optimized methods to 38 museum and private angler trophy specimens dating back to 1912 yielded sufficient DNA for downstream genomic analysis for 68% of the samples. No clear relationships between age of samples, DNA quality and quantity were observed, likely reflecting different preparation and storage methods for the trophies. Trial sequencing of DNA capture genomic libraries using 20,000 baits revealed that a significant proportion of captured sequences were derived from tiger sharks. This study demonstrates that archived shark jaws and vertebrae are potential high yield sources of DNA for genomic scale analysis. It also highlights that even for similar tissue types, a careful evaluation of extraction protocols can vastly improve DNA yield. This article is protected by copyright. All rights reserved. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 29% |
| Norway | 1 | 14% |
| United Kingdom | 1 | 14% |
| United States | 1 | 14% |
| Australia | 1 | 14% |
| Unknown | 1 | 14% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 57% |
| Scientists | 3 | 43% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Australia | 1 | 2% |
| Unknown | 46 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 19% |
| Student > Master | 8 | 17% |
| Researcher | 6 | 13% |
| Student > Bachelor | 4 | 8% |
| Student > Postgraduate | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 14 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 38% |
| Biochemistry, Genetics and Molecular Biology | 7 | 15% |
| Environmental Science | 5 | 10% |
| Computer Science | 1 | 2% |
| Earth and Planetary Sciences | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 15 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2018.
All research outputs
#7,944,450
of 24,593,555 outputs
Outputs from Molecular Ecology Resources
#1,006
of 1,741 outputs
Outputs of similar age
#117,482
of 344,781 outputs
Outputs of similar age from Molecular Ecology Resources
#27
of 41 outputs
Altmetric has tracked 24,593,555 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,741 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.5. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,781 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.