| Title |
Measurement of global DNA methylation levels by flow cytometry in mouse fibroblasts
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, August 2016
|
| DOI | 10.1007/s11626-016-0075-4 |
| Pubmed ID | |
| Authors |
Selcen Çelik-Uzuner, Yan Li, Lyndsay Peters, Chris O’Neill |
| Abstract |
DNA Methylation, 5meC, is an epigenetic modification that acts as an important regulator of genomic stability and gene expressivity. Genome-wide changes in methylation have been associated with lineage-specific changes in gene expression profiles during development and in some cell-based pathologies, including oncogenesis. Cost-effective and rapid platforms for the detection of changes in the global levels of methylation are of value for the investigation of the processes that regulate methylation. Flow cytometry allows rapid and quantitative analysis of epitopes within a large number of cells. We have recently optimised the conditions required for valid detection of 5meC by immunofluorescence microscopy. These studies showed that immunological detection of 5meC requires the sequential denaturation of chromatin by a brief period of acidification followed by a partial tryptic digestion step. We have assessed the reliability of flow cytometry for the detection of changes in 5meC when coupled with this optimised epitope retrieval strategy. This study provides support for the use of high throughput screening of 5meC by flow cytometry for the analysis of the epigenetic regulation of important cell transitions. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | 50% |
| France | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 23% |
| Student > Ph. D. Student | 6 | 23% |
| Student > Master | 3 | 12% |
| Student > Bachelor | 2 | 8% |
| Student > Doctoral Student | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 7 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Agricultural and Biological Sciences | 4 | 15% |
| Immunology and Microbiology | 3 | 12% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Nursing and Health Professions | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 7 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2016.
All research outputs
#15,329,969
of 22,882,389 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#484
of 793 outputs
Outputs of similar age
#232,895
of 361,768 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#2
of 10 outputs
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So far Altmetric has tracked 793 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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