| Title |
Hsp72 and Hsp90α mRNA transcription is characterised by large, sustained changes in core temperature during heat acclimation
|
|---|---|
| Published in |
Cell Stress and Chaperones, August 2016
|
| DOI | 10.1007/s12192-016-0726-0 |
| Pubmed ID | |
| Authors |
Oliver R Gibson, James A Tuttle, Peter W Watt, Neil S Maxwell, Lee Taylor |
| Abstract |
Increased intracellular heat shock protein-72 (Hsp72) and heat shock protein-90α (Hsp90α) have been implicated as important components of acquired thermotolerance, providing cytoprotection during stress. This experiment determined the physiological responses characterising increases in Hsp72 and Hsp90α mRNA on the first and tenth day of 90-min heat acclimation (in 40.2 °C, 41.0 % relative humidity (RH)) or equivalent normothermic training (in 20 °C, 29 % RH). Pearson's product-moment correlation and stepwise multiple regression were performed to determine relationships between physiological [e.g. (Trec, sweat rate (SR) and heart rate (HR)] and training variables (exercise duration, exercise intensity, work done), and the leukocyte Hsp72 and Hsp90α mRNA responses via reverse transcription quantitative polymerase chain reaction (RT-QPCR) (n = 15). Significant (p < 0.05) correlations existed between increased Hsp72 and Hsp90α mRNA (r = 0.879). Increased core temperature was the most important criteria for gene transcription with ΔTrec (r = 0.714), SR (r = 0.709), Trecfinal45 (r = 0.682), area under the curve where Trec ≥ 38.5 °C (AUC38.5 °C; r = 0.678), peak Trec (r = 0.661), duration Trec ≥ 38.5 °C (r = 0.650) and ΔHR (r = 0.511) each demonstrating a significant (p < 0.05) correlation with the increase in Hsp72 mRNA. The Trec AUC38.5 °C (r = 0.729), ΔTrec (r = 0.691), peak Trec (r = 0.680), Trecfinal45 (r = 0.678), SR (r = 0.660), duration Trec ≥ 38.5 °C (r = 0.629), the rate of change in Trec (r = 0.600) and ΔHR (r = 0.531) were the strongest correlate with the increase in Hsp90α mRNA. Multiple regression improved the model for Hsp90α mRNA only, when Trec AUC38.5 °C and SR were combined. Training variables showed insignificant (p > 0.05) weak (r < 0.300) relationships with Hsp72 and Hsp90α mRNA. Hsp72 and Hsp90α mRNA correlates were comparable on the first and tenth day. When transcription of the related Hsp72 and Hsp90α mRNA is important, protocols should rapidly induce large, prolonged changes in core temperature. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 5 | 50% |
| Australia | 1 | 10% |
| United States | 1 | 10% |
| Norway | 1 | 10% |
| Taiwan | 1 | 10% |
| Unknown | 1 | 10% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 40% |
| Scientists | 4 | 40% |
| Practitioners (doctors, other healthcare professionals) | 1 | 10% |
| Unknown | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Unknown | 78 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 22% |
| Student > Bachelor | 8 | 10% |
| Researcher | 7 | 9% |
| Student > Doctoral Student | 6 | 8% |
| Student > Master | 6 | 8% |
| Other | 14 | 18% |
| Unknown | 21 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Sports and Recreations | 23 | 29% |
| Medicine and Dentistry | 10 | 13% |
| Agricultural and Biological Sciences | 5 | 6% |
| Biochemistry, Genetics and Molecular Biology | 4 | 5% |
| Environmental Science | 3 | 4% |
| Other | 5 | 6% |
| Unknown | 29 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2019.
All research outputs
#6,760,834
of 25,394,764 outputs
Outputs from Cell Stress and Chaperones
#157
of 699 outputs
Outputs of similar age
#104,872
of 369,428 outputs
Outputs of similar age from Cell Stress and Chaperones
#6
of 13 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 699 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 77% of its peers.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.