| Title |
Increased riboflavin production by manipulation of inosine 5′-monophosphate dehydrogenase in Ashbya gossypii
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, July 2015
|
| DOI | 10.1007/s00253-015-6710-2 |
| Pubmed ID | |
| Authors |
Rubén M. Buey, Rodrigo Ledesma-Amaro, Mónica Balsera, José María de Pereda, José Luis Revuelta |
| Abstract |
Guanine nucleotides are the precursors of essential biomolecules including nucleic acids and vitamins such as riboflavin. The enzyme inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the ratelimiting step in the guanine nucleotide de novo biosynthetic pathway and plays a key role in controlling the cellular nucleotide pools. Thus, IMPDH is an important metabolic bottleneck in the guanine nucleotide synthesis, susceptible of manipulation by means of metabolic engineering approaches. Herein, we report the functional and structural characterization of the IMPDH enzyme from the industrial fungus Ashbya gossypii. Our data show that the overexpression of the IMPDH gene increases the metabolic flux through the guanine pathway and ultimately enhances 40 % riboflavin production with respect to the wild type. Also, IMPDH disruption results in a 100-fold increase of inosine excretion to the culture media. Our results contribute to the developing metabolic engineering toolbox aiming at improving the production of metabolites with biotechnological interest in A. gossypii. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 42 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 26% |
| Student > Master | 8 | 19% |
| Student > Ph. D. Student | 7 | 17% |
| Student > Bachelor | 3 | 7% |
| Lecturer | 1 | 2% |
| Other | 4 | 10% |
| Unknown | 8 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 48% |
| Agricultural and Biological Sciences | 7 | 17% |
| Chemistry | 2 | 5% |
| Immunology and Microbiology | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 8 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2016.
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#21,608,038
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#6,994
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#226,398
of 266,357 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#104
of 163 outputs
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