| Chapter title |
Isolating and Purifying Clostridium difficile Spores
|
|---|---|
| Chapter number | 9 |
| Book title |
Clostridium difficile
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6361-4_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6359-1, 978-1-4939-6361-4
|
| Authors |
Adrianne N. Edwards, Shonna M. McBride, Edwards, Adrianne N, McBride, Shonna M |
| Editors |
Adam P. Roberts, Peter Mullany |
| Abstract |
The ability for the obligate anaerobe, Clostridium difficile to form a metabolically dormant spore is critical for the survival of this organism outside of the host. This spore form is resistant to a myriad of environmental stresses, including heat, desiccation, and exposure to disinfectants and antimicrobials. These intrinsic properties of spores allow C. difficile to survive long-term in an oxygenated environment, to be easily transmitted from host-to-host, and to persist within the host following antibiotic treatment. Because of the importance of the spore form to the C. difficile life cycle and treatment and prevention of C. difficile infection (CDI), the isolation and purification of spores are necessary to study the mechanisms of sporulation and germination, investigate spore properties and resistances, and for use in animal models of CDI. Here we provide basic protocols, in vitro growth conditions, and additional considerations for purifying C. difficile spores for a variety of downstream applications. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 64 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 23% |
| Student > Bachelor | 7 | 11% |
| Student > Ph. D. Student | 7 | 11% |
| Professor > Associate Professor | 3 | 5% |
| Student > Master | 3 | 5% |
| Other | 6 | 9% |
| Unknown | 23 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 15 | 23% |
| Immunology and Microbiology | 12 | 19% |
| Agricultural and Biological Sciences | 4 | 6% |
| Veterinary Science and Veterinary Medicine | 2 | 3% |
| Environmental Science | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 27 | 42% |