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Mendeley readers
Attention Score in Context
| Title |
Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa
|
|---|---|
| Published in |
Animal Reproduction Science, September 2012
|
| DOI | 10.1016/j.anireprosci.2012.09.001 |
| Pubmed ID | |
| Authors |
Luis Anel-López, Manuel Álvarez-Rodríguez, Olga García-Álvarez, Mercedes Álvarez, Alejandro Maroto-Morales, Luis Anel, Paulino de Paz, J. Julián Garde, Felipe Martínez-Pastor |
| Abstract |
The use of assisted reproductive techniques in cervids is increasing as the commercial use of these species increase. We have tested the suitability of the antioxidants Trolox and reduced glutathione (GSH) for freezing red deer epididymal spermatozoa, aiming at improving post-thawing quality. Samples from 19 stags were frozen in a TES-Tris-fructose extender (20% egg yolk, 8% glycerol), with 1 or 5 mM of antioxidant. Motility (CASA), lipoperoxidation (malondialdehyde -MDA- production), membrane status, mitochondrial activity, acrosomal status (flow cytometry) and chromatin status (SCSA: %DFI and %HDS; flow cytometry) were assessed after thawing and after 6 h at 39°C. There were few differences between treatments after thawing, with Trolox reducing MDA production in a dose-response manner. After the incubation, sperm quality decreased and %DFI increased moderately, with no change for MDA. GSH improved motility, kinematic parameters and mitochondrial status, with a slight increase in %HDS. GSH 5 mM also increased moderately MDA production and %DFI, possibly due to enhanced metabolic activity and reducing power. Trolox maintained MDA low, but was detrimental to sperm quality. Trolox might not be appropriate for the cryopreservation of red deer epididymal spermatozoa, at least at the millimolar range. GSH results are promising, especially regarding motility improvement after the post-thawing incubation, and should be selected for future fertility trials. |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | 3% |
| Norway | 1 | 1% |
| Argentina | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 69 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 16% |
| Student > Doctoral Student | 9 | 12% |
| Professor | 8 | 11% |
| Student > Bachelor | 7 | 9% |
| Student > Ph. D. Student | 7 | 9% |
| Other | 17 | 23% |
| Unknown | 14 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 45% |
| Veterinary Science and Veterinary Medicine | 15 | 20% |
| Biochemistry, Genetics and Molecular Biology | 3 | 4% |
| Medicine and Dentistry | 3 | 4% |
| Computer Science | 1 | 1% |
| Other | 3 | 4% |
| Unknown | 16 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2012.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Animal Reproduction Science
#825
of 1,131 outputs
Outputs of similar age
#168,357
of 187,174 outputs
Outputs of similar age from Animal Reproduction Science
#7
of 11 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,131 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 187,174 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.