| Title |
Identification of Two Genes Encoding for the Late Acyltransferases of Lipid A in Klebsiella pneumoniae
|
|---|---|
| Published in |
Current Microbiology, August 2016
|
| DOI | 10.1007/s00284-016-1117-6 |
| Pubmed ID | |
| Authors |
Yanyan Li, Jian Yun, Lu Liu, Ye Li, Xiaoyuan Wang |
| Abstract |
Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most gram-negative bacteria. It is recognized by the TLR4/MD2 receptor of the innate immune system, which triggers an inflammatory response accompanied by massive overproduction of cytokines and leads to gram-negative septic shock. Human pathogen Klebsiella pneumoniae, which may synthesize two lipid A species, differs by the length of the secondary acyl chain. In this study, we identified two genes encoding the putative late acyltransferases of lipid A biosynthesis pathway in K. pneumoniae. Based on the sequence alignment, proteins YP_002239312.1 encoded by KPK3489 and YP_002239899.1 encoded by KPK4096 are homologous to E. coli LpxL, which were designated as LpxL1 and LpxL2, respectively. Functions of the two acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid A and analyzing their structures using an ESI/MS. Like E. coli LpxL, K. pneumoniae LpxL1 transfers a C12:0 secondary acyl chain to the 2'-position of lipid A, while K. pneumoniae LpxL2 transfers a C14:0 secondary acyl chain to the 2'-position primary acyl chain of lipid A. These two acyltransferases might play important roles in the biosynthesis of lipid A and the innate immune system recognition in K. pneumoniae. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 29% |
| Student > Ph. D. Student | 3 | 21% |
| Student > Bachelor | 1 | 7% |
| Student > Master | 1 | 7% |
| Unknown | 5 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 21% |
| Immunology and Microbiology | 3 | 21% |
| Medicine and Dentistry | 2 | 14% |
| Agricultural and Biological Sciences | 1 | 7% |
| Business, Management and Accounting | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 August 2016.
All research outputs
#20,337,788
of 22,883,326 outputs
Outputs from Current Microbiology
#1,919
of 2,415 outputs
Outputs of similar age
#299,060
of 342,741 outputs
Outputs of similar age from Current Microbiology
#19
of 27 outputs
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