| Title |
In vitro anti-hepatitis B virus effect of Hypericum perforatum L.
|
|---|---|
| Published in |
Current Medical Science, February 2010
|
| DOI | 10.1007/s11596-010-0118-0 |
| Pubmed ID | |
| Authors |
Ran Pang, Junyan Tao, Shuling Zhang, Jiang Zhu, Xin Yue, Lei Zhao, Pian Ye, Ying Zhu |
| Abstract |
The anti-hepatitis B virus (HBV) effects and its mechanisms of the ethanol extracts of Hypericum perforatum L. (EHP) in vitro were explored. HepG2 2.2.15 cells, a stable HBV-producing cell line, were cultured as the model system to observe the anti-HBV effect. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA). The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR. In order to understand the mechanisms of the suppression of HBV replication, all HBV promoters (Cp, Xp, S1p, S2p and Fp) with luciferase reporter gene were transfected into HepG2 cells respectively. Then the activities of viral promoters were examined by luciferase reporter assay. It was found EHP effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose-dependent manner, as well as the extracellular HBV DNA. And EHP could selectively inhibit the activity of HBV promoter Fp. Our data suggest that EHP exerts anti-HBV effects via inhibition of HBV transcription, which helps to elucidate the mechanism underlying the potential therapeutic value of EHP. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ireland | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 5% |
| Unknown | 21 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 18% |
| Student > Ph. D. Student | 3 | 14% |
| Student > Bachelor | 3 | 14% |
| Student > Doctoral Student | 2 | 9% |
| Lecturer | 2 | 9% |
| Other | 6 | 27% |
| Unknown | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 18% |
| Medicine and Dentistry | 2 | 9% |
| Immunology and Microbiology | 2 | 9% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Other | 7 | 32% |
| Unknown | 1 | 5% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 November 2016.
All research outputs
#14,915,133
of 25,374,917 outputs
Outputs from Current Medical Science
#265
of 719 outputs
Outputs of similar age
#140,839
of 176,072 outputs
Outputs of similar age from Current Medical Science
#3
of 3 outputs
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So far Altmetric has tracked 719 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 63% of its peers.
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