Title |
Increased Protein-Coding Mutations in the Mitochondrial Genome of African American Women With Preeclampsia
|
---|---|
Published in |
Reproductive Sciences, December 2012
|
DOI | 10.1177/1933719112450337 |
Pubmed ID | |
Authors |
David Ding, Nicole M. Scott, Emma E. Thompson, Tinnakorn Chaiworapongsa, Raul Torres, Christine Billstrand, Kathleen Murray, Phillip J. Dexheimer, Mahmoud Ismail, Helen Kay, Shawn Levy, Roberto Romero, Marshall D. Lindheimer, Dan L. Nicolae, Carole Ober |
Abstract |
Preeclampsia occurs more frequently in women of African ancestry. The cause of this hypertensive complication is unclear, but placental oxidative stress may play a role. Because mitochondria are the major sites of oxidative phosphorylation, we hypothesized that placentas of preeclamptic pregnancies harbor mitochondrial DNA (mtDNA) mutations. Next-generation sequencing of placental mtDNA in African American preeclamptics (N = 30) and controls (N = 38) from Chicago revealed significant excesses in preeclamptics of nonsynonymous substitutions in protein-coding genes and mitochondrially encoded nicotinamide adenine dinucleotide dehydrogenase 5 gene and an increase in the substitution rate (P = .0001). Moreover, 88% of preeclamptics and 53% of controls carried at least one nonsynonymous substitution (P = .005; odds ratio [OR] = 6.36, 95% confidence interval [CI]: 1.5-39.1). These results were not replicated in a sample of African American preeclamptics (N = 162) and controls (N = 171) from Detroit. Differences in study design and heterogeneity may account for this lack of replication. Nonsynonymous substitutions in mtDNA may be risk factors for preeclampsia in some African American women, but additional studies are required to establish this relationship. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 21% |
Professor | 6 | 18% |
Student > Bachelor | 4 | 12% |
Student > Ph. D. Student | 3 | 9% |
Student > Postgraduate | 2 | 6% |
Other | 6 | 18% |
Unknown | 6 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 12 | 35% |
Biochemistry, Genetics and Molecular Biology | 6 | 18% |
Agricultural and Biological Sciences | 5 | 15% |
Unspecified | 1 | 3% |
Linguistics | 1 | 3% |
Other | 3 | 9% |
Unknown | 6 | 18% |