| Title |
Combined Infrared Multiphoton Dissociation with Ultraviolet Photodissociation for Ubiquitin Characterization
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, June 2016
|
| DOI | 10.1007/s13361-016-1419-8 |
| Pubmed ID | |
| Authors |
Mohammad A. Halim, Marion Girod, Luke MacAleese, Jérôme Lemoine, Rodolphe Antoine, Philippe Dugourd |
| Abstract |
Herein we report the successful implementation of the consecutive and simultaneous photodissociation with high (213 nm) and low (10.6 μm) energy photons (HiLoPD, high-low photodissociation) on ubiquitin in a quadrupole-Orbitrap mass spectrometer. Absorption of high-energy UV photon is dispersed over the whole protein and stimulates extensive C-Cα backbone fragmentation, whereas low-energy IR photon gradually increases the internal energy and thus preferentially dissociates the most labile amide (C-N) bonds. We noticed that simultaneous irradiation of UV and IR lasers on intact ubiquitin in a single MS/MS experiment provides a rich and well-balanced fragmentation array of a/x, b/y, and z ions. Moreover, secondary fragmentation from a/x and z ions leads to the formation of satellite side-chain ions (d, v, and w) and can help to distinguish isomeric residues in a protein. Implementation of high-low photodissociation in a high-resolution mass spectrometer may offer considerable benefits to promote a comprehensive portrait of protein characterization. Graphical Abstract ᅟ. |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 66 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 27% |
| Researcher | 10 | 15% |
| Student > Master | 6 | 9% |
| Professor | 6 | 9% |
| Student > Bachelor | 4 | 6% |
| Other | 13 | 20% |
| Unknown | 9 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 36 | 55% |
| Biochemistry, Genetics and Molecular Biology | 7 | 11% |
| Physics and Astronomy | 3 | 5% |
| Agricultural and Biological Sciences | 2 | 3% |
| Computer Science | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 12 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 August 2016.
All research outputs
#6,604,748
of 25,394,764 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#881
of 3,836 outputs
Outputs of similar age
#100,605
of 360,231 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#4
of 26 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 3,836 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,231 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.