Chapter title |
Chromosome and Genomic Engineering in Plants
|
---|---|
Chapter number | 11 |
Book title |
Chromosome and Genomic Engineering in Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4931-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4929-8, 978-1-4939-4931-1
|
Authors |
Kobayashi, Takehito, Yagi, Yusuke, Nakamura, Takahiro, Takehito Kobayashi, Yusuke Yagi, Takahiro Nakamura |
Abstract |
The pentatricopeptide repeat (PPR) motif is a sequence-specific RNA/DNA-binding module. Elucidation of the RNA/DNA recognition mechanism has enabled engineering of PPR motifs as new RNA/DNA manipulation tools in living cells, including for genome editing. However, the biochemical characteristics of PPR proteins remain unknown, mostly due to the instability and/or unfolding propensities of PPR proteins in heterologous expression systems such as bacteria and yeast. To overcome this issue, we constructed reporter systems using animal cultured cells. The cell-based system has highly attractive features for PPR engineering: robust eukaryotic gene expression; availability of various vectors, reagents, and antibodies; highly efficient DNA delivery ratio (>80 %); and rapid, high-throughput data production. In this chapter, we introduce an example of such reporter systems: a PPR-based sequence-specific translational activation system. The cell-based reporter system can be applied to characterize plant genes of interested and to PPR engineering. |
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France | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 11 | 100% |
Demographic breakdown
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Researcher | 3 | 27% |
Student > Doctoral Student | 2 | 18% |
Professor | 1 | 9% |
Student > Master | 1 | 9% |
Student > Postgraduate | 1 | 9% |
Other | 0 | 0% |
Unknown | 3 | 27% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 36% |
Agricultural and Biological Sciences | 2 | 18% |
Social Sciences | 1 | 9% |
Chemistry | 1 | 9% |
Unknown | 3 | 27% |