| Title |
“Vacuum-assisted staining”: a simple and efficient method for screening in Drosophila
|
|---|---|
| Published in |
Development Genes and Evolution, February 2012
|
| DOI | 10.1007/s00427-012-0391-y |
| Pubmed ID | |
| Authors |
Nicola Berns, Innokenty Woichansky, Nadine Kraft, Ulrike Hüsken, Matthias Carl, Veit Riechmann |
| Abstract |
The constantly growing number of genetic tools rapidly increases possibilities for various screens in different model organisms and calls for new methods facilitating screen performance. In particular, screening procedures involving fixation and staining of samples are difficult to perform at a genome-wide scale. The time-consuming task to generate these samples makes such screens less attractive. Here, we describe the use of multi-well filter plates for high throughput labellings of different Drosophila organs and zebrafish embryos. Our inexpensive vacuum-assisted staining protocol minimises the risk of sample loss, reduces the amount of staining reagents and drastically decreases labour and repetitive work. The simple handling of the system and the commercial availability of its components makes this method easily applicable to every laboratory. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 38% |
| Student > Ph. D. Student | 3 | 23% |
| Student > Bachelor | 2 | 15% |
| Student > Doctoral Student | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 77% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Economics, Econometrics and Finance | 1 | 8% |
| Neuroscience | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2012.
All research outputs
#19,221,261
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Outputs from Development Genes and Evolution
#427
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#123,005
of 157,720 outputs
Outputs of similar age from Development Genes and Evolution
#1
of 1 outputs
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