| Title |
Bioconversion of Agave tequilana fructans by exo-inulinases from indigenous Aspergillus niger CH-A-2010 enhances ethanol production from raw Agave tequilana juice
|
|---|---|
| Published in |
Journal of Industrial Microbiology & Biotechnology, January 2013
|
| DOI | 10.1007/s10295-012-1211-0 |
| Pubmed ID | |
| Authors |
Carlos Huitrón, Rosalba Pérez, Luís Gutiérrez, Patricia Lappe, Pavel Petrosyan, Jesús Villegas, Cecilia Aguilar, Leticia Rocha-Zavaleta, Abel Blancas |
| Abstract |
Agave tequilana fructans are the source of fermentable sugars for the production of tequila. Fructans are processed by acid hydrolysis or by cooking in ovens at high temperature. Enzymatic hydrolysis is considered an alternative for the bioconversion of fructans. We previously described the isolation of Aspergillus niger CH-A-2010, an indigenous strain that produces extracellular inulinases. Here we evaluated the potential application of A. niger CH-A-2010 inulinases for the bioconversion of A. tequilana fructans, and its impact on the production of ethanol. Inulinases were analyzed by Western blotting and thin layer chromatography. Optimal pH and temperature conditions for inulinase activity were determined. The efficiency of A. niger CH-A-2010 inulinases was compared with commercial enzymes and with acid hydrolysis. The hydrolysates obtained were subsequently fermented by Saccharomyces cerevisiae to determine the efficiency of ethanol production. Results indicate that A. niger CH-A-2010 predominantly produces an exo-inulinase activity. Optimal inulinase activity occurred at pH 5.0 and 50 °C. Hydrolysis of raw agave juice by CH-A-2010 inulinases yielded 33.5 g/l reducing sugars, compared with 27.3 g/l by Fructozyme(®) (Novozymes Corp, Bagsværd, Denmark) and 29.4 g/l by acid hydrolysis. After fermentation of hydrolysates, we observed that the conversion efficiency of sugars into ethanol was 97.5 % of the theoretical ethanol yield for enzymatically degraded agave juice, compared to 83.8 % for acid-hydrolyzed juice. These observations indicate that fructans from raw Agave tequilana juice can be efficiently hydrolyzed by using A. niger CH-A-2010 inulinases, and that this procedure impacts positively on the production of ethanol. |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 2 | 3% |
| Unknown | 63 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 17% |
| Researcher | 10 | 15% |
| Student > Ph. D. Student | 9 | 14% |
| Student > Doctoral Student | 5 | 8% |
| Student > Bachelor | 4 | 6% |
| Other | 16 | 25% |
| Unknown | 10 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 43% |
| Biochemistry, Genetics and Molecular Biology | 6 | 9% |
| Nursing and Health Professions | 2 | 3% |
| Immunology and Microbiology | 2 | 3% |
| Chemical Engineering | 2 | 3% |
| Other | 13 | 20% |
| Unknown | 12 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2012.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Journal of Industrial Microbiology & Biotechnology
#1,453
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Outputs of similar age
#258,406
of 288,986 outputs
Outputs of similar age from Journal of Industrial Microbiology & Biotechnology
#12
of 13 outputs
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