| Chapter title |
Laser Capture Microdissection as a Tool to Study Tumor Stroma.
|
|---|---|
| Chapter number | 2 |
| Book title |
The Tumor Microenvironment
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3801-8_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3799-8, 978-1-4939-3801-8
|
| Authors |
Bertos, Nicholas R, Park, Morag, Nicholas R. Bertos, Morag Park |
| Editors |
Josie Ursini-Siegel, Nicole Beauchemin |
| Abstract |
Laser capture microdissection (or LCM) allows for isolation of cells from specific tissue compartments, which can then be followed by DNA, RNA, and/or protein isolation and downstream characterization. Unlike other methods for cell isolation, LCM can be directed towards cells situated in specific anatomical contexts, and is therefore of significant value when investigating the tumor microenvironment, where localization is often key to function. Here, we present a summary of ways in which LCM can be utilized, as well as protocols for the isolation of tumor and tumor-associated stromal elements from frozen breast cancer samples, with a focus on preparation of samples for RNA characterization. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 20% |
| Professor > Associate Professor | 1 | 20% |
| Researcher | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Medicine and Dentistry | 1 | 20% |
| Engineering | 1 | 20% |
| Unknown | 1 | 20% |