| Title |
Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII selection
|
|---|---|
| Published in |
Plant Cell Reports, November 2012
|
| DOI | 10.1007/s00299-012-1366-1 |
| Pubmed ID | |
| Authors |
Christopher J. Pantazis, Sarah Fisk, Kerri Mills, Barry S. Flinn, Vladimir Shulaev, Richard E. Veilleux, Yinghui Dan |
| Abstract |
KEY MESSAGE : We developed an efficient Agrobacterium -mediated transformation method using an Ac/Ds transposon tagging construct for F. vesca and high throughput paromomycin spray assay to identify its transformants for strawberry functional genomics. Genomic resources for Rosaceae species are now readily available, including the Fragaria vesca genome, EST sequences, markers, linkage maps, and physical maps. The Rosaceae Genomic Executive Committee has promoted strawberry as a translational genomics model due to its unique biological features and transformability for fruit trait improvement. Our overall research goal is to use functional genomic and metabolic approaches to pursue high throughput gene discovery in the diploid woodland strawberry. F. vesca offers several advantages of a fleshy fruit typical of most fruit crops, short life cycle (seed to seed in 12-16 weeks), small genome size (206 Mbb/C), small plant size, self-compatibility, and many seeds per plant. We have developed an efficient Agrobacterium tumefaciens-mediated strawberry transformation method using kanamycin selection, and high throughput paromomycin spray assay to efficiently identify transgenic strawberry plants. Using our kanamycin transformation method, we were able to produce up to 98 independent kanamycin resistant insertional mutant lines using a T-DNA construct carrying an Ac/Ds transposon Launchpad system from a single transformation experiment involving inoculation of 22 leaf explants of F. vesca accession 551572 within approx. 11 weeks (from inoculation to soil). Transgenic plants with 1-2 copies of a transgene were confirmed by Southern blot analysis. Using our paromomycin spray assay, transgenic F. vesca plants were rapidly identified within 10 days after spraying. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 17% |
| Student > Ph. D. Student | 6 | 13% |
| Student > Doctoral Student | 6 | 13% |
| Student > Master | 6 | 13% |
| Student > Bachelor | 5 | 11% |
| Other | 8 | 17% |
| Unknown | 8 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 57% |
| Biochemistry, Genetics and Molecular Biology | 8 | 17% |
| Engineering | 2 | 4% |
| Medicine and Dentistry | 1 | 2% |
| Mathematics | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 8 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2012.
All research outputs
#18,323,689
of 22,689,790 outputs
Outputs from Plant Cell Reports
#1,879
of 2,177 outputs
Outputs of similar age
#121,193
of 159,113 outputs
Outputs of similar age from Plant Cell Reports
#4
of 4 outputs
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