| Title |
Genomewide analysis indicates that queen larvae have lower methylation levels in the honey bee (Apis mellifera)
|
|---|---|
| Published in |
The Science of Nature, December 2012
|
| DOI | 10.1007/s00114-012-1004-3 |
| Pubmed ID | |
| Authors |
Yuan Yuan Shi, Wei Yu Yan, Zachary Y. Huang, Zi Long Wang, Xiao Bo Wu, Zhi Jiang Zeng |
| Abstract |
The honey bee is a social insect characterized by caste differentiation, by which a young larva can develop into either a queen or a worker. Despite possessing the same genome, queen and workers display marked differences in reproductive capacity, physiology, and behavior. Recent studies have shown that DNA methylation plays important roles in caste differentiation. To further explore the roles of DNA methylation in this process, we analyzed DNA methylome profiles of both queen larvae (QL) and worker larvae (WL) of different ages (2, 4, and 6 day old), by using methylated DNA immunoprecipitation-sequencing (meDIP-seq) technique. The global DNA methylation levels varied between the larvae of two castes. DNA methylation increased from 2-day- to 4-day-old QL and then decreased in 6-day-old larvae. In WL, methylation levels increased with age. The methylcytosines in both larvae were enriched in introns, followed by coding sequence (CDS) regions, CpG islands, 2 kbp downstream and upstream of genes, and 5' and 3' untranslated regions (UTRs). The number of differentially methylated genes (DMGs) in 2-, 4-, and 6-day-old QL and WL was 725, 3,013, and 5,049, respectively. Compared to 4- and 6-day-old WL, a large number of genes in QL were downmethylated, which were involved in many processes including development, reproduction, and metabolic regulation. In addition, some DMGs were concerned with caste differentiation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 3 | 4% |
| Japan | 1 | 1% |
| United States | 1 | 1% |
| Luxembourg | 1 | 1% |
| Unknown | 65 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 23 | 32% |
| Researcher | 15 | 21% |
| Student > Master | 6 | 8% |
| Student > Doctoral Student | 5 | 7% |
| Student > Bachelor | 4 | 6% |
| Other | 6 | 8% |
| Unknown | 12 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 39 | 55% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Nursing and Health Professions | 1 | 1% |
| Environmental Science | 1 | 1% |
| Earth and Planetary Sciences | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 18 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2022.
All research outputs
#3,264,545
of 25,837,817 outputs
Outputs from The Science of Nature
#385
of 2,282 outputs
Outputs of similar age
#30,584
of 289,772 outputs
Outputs of similar age from The Science of Nature
#4
of 13 outputs
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