| Title |
Nutrigenomic analysis of the protective effects of bilberry anthocyanin-rich extract in apo E-deficient mice
|
|---|---|
| Published in |
Genes & Nutrition, March 2010
|
| DOI | 10.1007/s12263-010-0171-0 |
| Pubmed ID | |
| Authors |
Aurelie Mauray, Catherine Felgines, Christine Morand, Andrzej Mazur, Augustin Scalbert, Dragan Milenkovic |
| Abstract |
Intake of anthocyanin-rich foods has been associated with a reduced risk of cardiovascular diseases. Supplementation with anthocyanin-rich extracts from black rice or purple sweet potato was reported to attenuate atherosclerotic lesion development in apolipoprotein E-deficient (apo E(-/-)) mice. However, the mechanism(s) of their preventive action are not completely understood. Previous studies revealed that anthocyanins altered mRNA levels of genes related to atherosclerosis in cultured macrophages and endothelial cells, but in vivo studies remain scarce. The aim of the study was to investigate the impact of bilberry anthocyanin-rich extract (BE) supplementation on gene expression in the liver of apo E(-/-) mice, the widely used model of atherosclerosis. The liver was chosen because it is the main site of lipid metabolism. Apo E(-/-) mice received for 2 weeks a standard diet supplemented with a nutritional dose of BE (0.02%). This study focused on the early stage of atherosclerosis development for better assessment of anthocyanin action on initiation mechanisms of this pathology. The results showed that a 2-week supplementation significantly reduced plasmatic total cholesterol and hepatic triglyceride levels, whereas the plasmatic antioxidant status remained unchanged. Transcriptional analysis, using microarrays, revealed that the expression of 2,289 genes was significantly altered. BE over-expressed genes involved in bile acid synthesis and cholesterol uptake into the liver and down-regulated the expression of pro-inflammatory genes. These results suggest an anti-atherogenic effect of BE through the regulation of cholesterol metabolism and liver inflammation and provide a global integrated view of the mechanisms involved in the preventive action of this extract. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Indonesia | 1 | 2% |
| Mexico | 1 | 2% |
| Netherlands | 1 | 2% |
| Belgium | 1 | 2% |
| Unknown | 61 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 18% |
| Researcher | 11 | 17% |
| Student > Master | 7 | 11% |
| Student > Doctoral Student | 5 | 8% |
| Student > Bachelor | 4 | 6% |
| Other | 11 | 17% |
| Unknown | 15 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 22% |
| Medicine and Dentistry | 11 | 17% |
| Biochemistry, Genetics and Molecular Biology | 7 | 11% |
| Chemistry | 4 | 6% |
| Engineering | 4 | 6% |
| Other | 9 | 14% |
| Unknown | 16 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2012.
All research outputs
#15,258,711
of 22,689,790 outputs
Outputs from Genes & Nutrition
#240
of 387 outputs
Outputs of similar age
#76,850
of 94,008 outputs
Outputs of similar age from Genes & Nutrition
#2
of 2 outputs
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