Title |
Nuclear Reprogramming with c-Myc Potentiates Glycolytic Capacity of Derived Induced Pluripotent Stem Cells
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Published in |
Journal of Cardiovascular Translational Research, December 2012
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DOI | 10.1007/s12265-012-9431-2 |
Pubmed ID | |
Authors |
Clifford D. L. Folmes, Almudena Martinez-Fernandez, Randolph S. Faustino, Satsuki Yamada, Carmen Perez-Terzic, Timothy J. Nelson, Andre Terzic |
Abstract |
Reprogramming strategies influence the differentiation capacity of derived induced pluripotent stem (iPS) cells. Removal of the reprogramming factor c-Myc reduces tumorigenic incidence and increases cardiogenic potential of iPS cells. c-Myc is a regulator of energy metabolism, yet the impact on metabolic reprogramming underlying pluripotent induction is unknown. Here, mitochondrial and metabolic interrogation of iPS cells derived with (4F) and without (3F) c-Myc demonstrated that nuclear reprogramming consistently reverted mitochondria to embryonic-like immature structures. Metabolomic profiling segregated derived iPS cells from the parental somatic source based on the attained pluripotency-associated glycolytic phenotype and discriminated between 3F versus 4F clones based upon glycolytic intermediates. Real-time flux analysis demonstrated a greater glycolytic capacity in 4F iPS cells, in the setting of equivalent oxidative capacity to 3F iPS cells. Thus, inclusion of c-Myc potentiates the pluripotent glycolytic behavior of derived iPS cells, supporting c-Myc-free reprogramming as a strategy to facilitate oxidative metabolism-dependent lineage engagement. |
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Mendeley readers
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Czechia | 1 | 2% |
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Unknown | 58 | 97% |
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Researcher | 15 | 25% |
Other | 4 | 7% |
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Professor | 3 | 5% |
Other | 4 | 7% |
Unknown | 13 | 22% |
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Unspecified | 1 | 2% |
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