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RNA-Guided Human Genome Engineering via Cas9

Overview of attention for article published in Science, January 2013
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Citations

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7847 Dimensions

Readers on

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9717 Mendeley
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17 CiteULike
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Title
RNA-Guided Human Genome Engineering via Cas9
Published in
Science, January 2013
DOI 10.1126/science.1232033
Pubmed ID
Authors

Prashant Mali, Luhan Yang, Kevin M. Esvelt, John Aach, Marc Guell, James E. DiCarlo, Julie E. Norville, George M. Church

Abstract

Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that use short RNA to direct degradation of foreign nucleic acids. Here, we engineer the type II bacterial CRISPR system to function with custom guide RNA (gRNA) in human cells. For the endogenous AAVS1 locus, we obtained targeting rates of 10 to 25% in 293T cells, 13 to 8% in K562 cells, and 2 to 4% in induced pluripotent stem cells. We show that this process relies on CRISPR components; is sequence-specific; and, upon simultaneous introduction of multiple gRNAs, can effect multiplex editing of target loci. We also compute a genome-wide resource of ~190 K unique gRNAs targeting ~40.5% of human exons. Our results establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.

X Demographics

X Demographics

The data shown below were collected from the profiles of 98 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9,717 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 110 1%
United Kingdom 37 <1%
Germany 29 <1%
Japan 18 <1%
France 16 <1%
Canada 14 <1%
Spain 12 <1%
China 12 <1%
Netherlands 11 <1%
Other 95 <1%
Unknown 9363 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2287 24%
Researcher 1577 16%
Student > Bachelor 1476 15%
Student > Master 1235 13%
Student > Doctoral Student 434 4%
Other 1214 12%
Unknown 1494 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 3524 36%
Biochemistry, Genetics and Molecular Biology 2729 28%
Medicine and Dentistry 505 5%
Neuroscience 231 2%
Engineering 209 2%
Other 848 9%
Unknown 1671 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 693. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2024.
All research outputs
#30,308
of 25,559,053 outputs
Outputs from Science
#1,326
of 83,117 outputs
Outputs of similar age
#125
of 289,849 outputs
Outputs of similar age from Science
#4
of 707 outputs
Altmetric has tracked 25,559,053 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,117 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 65.8. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 289,849 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 707 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.