Title |
Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells
|
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Published in |
Methods in Cell Science, April 2009
|
DOI | 10.1007/s10616-009-9191-2 |
Pubmed ID | |
Authors |
Junkyu Han, Terence P. N. Talorete, Parida Yamada, Hiroko Isoda |
Abstract |
Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxytyrosol. In this study, oleuropein and hydroxytyrosol, major phenolic compound of olive oil, was studied for its effects on growth in MCF-7 human breast cancer cells using assays for proliferation (MTT assay), cell viability (Guava ViaCount assay), cell apoptosis, cellcycle (flow cytometry). Oleuropein or hydroxytyrosol decreased cell viability, inhibited cell proliferation, and induced cell apoptosis in MCF-7 cells. Result of MTT assay showed that 200 mug/mL of oleuropein or 50 mug/mL of hydroxytyrosol remarkably reduced cell viability of MCF-7 cells. Oleuropein or hydroxytyrosol decrease of the number of MCF-7 cells by inhibiting the rate of cell proliferation and inducing cell apoptosis. Also hydroxytyrosol and oleuropein exhibited statistically significant block of G(1) to S phase transition manifested by the increase of cell number in G(0)/G(1) phase. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | <1% |
Chile | 1 | <1% |
Brazil | 1 | <1% |
Japan | 1 | <1% |
Mexico | 1 | <1% |
Croatia | 1 | <1% |
Poland | 1 | <1% |
Unknown | 229 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 37 | 16% |
Student > Ph. D. Student | 35 | 15% |
Student > Bachelor | 33 | 14% |
Student > Master | 32 | 14% |
Student > Doctoral Student | 16 | 7% |
Other | 34 | 14% |
Unknown | 50 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 61 | 26% |
Biochemistry, Genetics and Molecular Biology | 38 | 16% |
Chemistry | 18 | 8% |
Medicine and Dentistry | 17 | 7% |
Engineering | 8 | 3% |
Other | 28 | 12% |
Unknown | 67 | 28% |