| Title |
Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and induce apoptosis in cervical cancer cells
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, September 2016
|
| DOI | 10.1186/s12906-016-1280-0 |
| Pubmed ID | |
| Authors |
Sindiswa T. Lukhele, Lesetja R. Motadi |
| Abstract |
Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year. Different therapeutic regimens have been suggested in various regions of Africa, however, over a quarter of a million women die of cervical cancer, annually. This makes it the most lethal cancer amongst black women and calls for urgent therapeutic strategies. In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines. To achieve our aim, phytochemical screening, MTT assay, cell growth analysis, flow cytometry, morphology analysis, Western blot, caspase 3/7 assay, and ATP measurement assay were conducted. Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. They further revealed that apoptosis was induced by cannabidiol as shown by increased subG0/G1 and apoptosis through annexin V. Apoptosis was confirmed by overexpression of p53, caspase 3 and bax. Apoptosis induction was further confirmed by morphological changes, an increase in Caspase 3/7 and a decrease in the ATP levels. In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. |
X Demographics
The data shown below were collected from the profiles of 126 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 44 | 35% |
| Canada | 13 | 10% |
| United Kingdom | 7 | 6% |
| South Africa | 6 | 5% |
| Australia | 4 | 3% |
| Ireland | 1 | <1% |
| France | 1 | <1% |
| Argentina | 1 | <1% |
| Spain | 1 | <1% |
| Other | 6 | 5% |
| Unknown | 42 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 115 | 91% |
| Practitioners (doctors, other healthcare professionals) | 6 | 5% |
| Scientists | 4 | 3% |
| Science communicators (journalists, bloggers, editors) | 1 | <1% |
Mendeley readers
The data shown below were compiled from readership statistics for 259 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | <1% |
| Colombia | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 255 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 37 | 14% |
| Student > Master | 35 | 14% |
| Researcher | 30 | 12% |
| Student > Ph. D. Student | 23 | 9% |
| Unspecified | 21 | 8% |
| Other | 49 | 19% |
| Unknown | 64 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 39 | 15% |
| Medicine and Dentistry | 32 | 12% |
| Agricultural and Biological Sciences | 21 | 8% |
| Unspecified | 21 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 21 | 8% |
| Other | 52 | 20% |
| Unknown | 73 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 422. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2024.
All research outputs
#69,163
of 25,610,986 outputs
Outputs from BMC Complementary Medicine and Therapies
#9
of 3,977 outputs
Outputs of similar age
#1,542
of 349,055 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#2
of 101 outputs
Altmetric has tracked 25,610,986 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,977 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 349,055 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 101 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.