| Title |
Screening and characterization of early diagnostic antigens in excretory–secretory proteins from Trichinella spiralis intestinal infective larvae by immunoproteomics
|
|---|---|
| Published in |
Parasitology Research, October 2015
|
| DOI | 10.1007/s00436-015-4779-2 |
| Pubmed ID | |
| Authors |
Ruo Dan Liu, Peng Jiang, Hui Wen, Jiang Yang Duan, Li Ang Wang, Jie Feng Li, Chun Ying Liu, Ge Ge Sun, Zhong Quan Wang, Jing Cui |
| Abstract |
The excretory-secretory (ES) antigens from Trichinella spiralis muscle larvae are the most commonly used diagnostic antigens for trichinellosis, but specific IgG antibodies were not detected in early stage of infection. The aim of this study was to identify early diagnostic antigens from ES proteins of intestinal infective larvae (IIL), the first invasive stage of T. spiralis. Six bands (92, 52, 45, 35, 32, and 29 kDa) of IIL ES proteins were recognized by infection sera in Western blotting as early as 10 days post infection. Total of 54 T. spiralis proteins in six bands were identified by shotgun LC-MS/MS, 30 proteins were annotated, and 27 had hydrolase activity. Several proteins (serine protease, putative trypsin, deoxyribonuclease II family protein, etc.) could be considered as the potential early diagnostic antigens for trichinellosis. Our study provides new insights for screening early diagnostic antigens from intestinal worms of T. spiralis. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 20% |
| Student > Ph. D. Student | 2 | 20% |
| Unspecified | 1 | 10% |
| Student > Postgraduate | 1 | 10% |
| Other | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 20% |
| Unspecified | 1 | 10% |
| Energy | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 5 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2016.
All research outputs
#17,814,957
of 22,886,568 outputs
Outputs from Parasitology Research
#2,085
of 3,791 outputs
Outputs of similar age
#188,132
of 279,314 outputs
Outputs of similar age from Parasitology Research
#54
of 130 outputs
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