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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Analyzing Cell Death by Nuclear Staining with Hoechst 33342.
|
|---|---|
| Published in |
Cold Spring Harbor Protocols, September 2016
|
| DOI | 10.1101/pdb.prot087205 |
| Pubmed ID | |
| Authors |
Lisa C Crowley, Brooke J Marfell, Nigel J Waterhouse |
| Abstract |
The nuclei of healthy cells are generally spherical, and the DNA is evenly distributed. During apoptosis the DNA becomes condensed, but this process does not occur during necrosis. Nuclear condensation can therefore be used to distinguish apoptotic cells from healthy cells or necrotic cells. Dyes that bind to DNA, such as Hoechst 33342 or 4',6-diamidino-2-phenylindole (DAPI), can be used to observe nuclear condensation. These dyes fluoresce at 461 nm when excited by ultraviolet light and can therefore be visualized using conventional fluorescent microscopes equipped with light sources that emit light at ∼350 nm and filter sets that permit the transmission of light at ∼460 nm. This protocol describes staining and visualization of cells stained with Hoechst 33342, but it can be adapted for staining with DAPI or other dyes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 309 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 309 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 47 | 15% |
| Student > Bachelor | 45 | 15% |
| Student > Master | 35 | 11% |
| Researcher | 20 | 6% |
| Student > Doctoral Student | 14 | 5% |
| Other | 47 | 15% |
| Unknown | 101 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 63 | 20% |
| Agricultural and Biological Sciences | 23 | 7% |
| Chemistry | 17 | 6% |
| Medicine and Dentistry | 16 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 15 | 5% |
| Other | 62 | 20% |
| Unknown | 113 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 September 2016.
All research outputs
#18,469,995
of 22,886,568 outputs
Outputs from Cold Spring Harbor Protocols
#622
of 972 outputs
Outputs of similar age
#258,239
of 337,395 outputs
Outputs of similar age from Cold Spring Harbor Protocols
#21
of 33 outputs
Altmetric has tracked 22,886,568 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 972 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 337,395 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.