| Title |
Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2013
|
| DOI | 10.1007/s00253-012-4685-9 |
| Pubmed ID | |
| Authors |
P. Jyothsna, Q. M. I. Haq, Priyanka Singh, K. V. Sumiya, Shelly Praveen, Ramaveer Rawat, Rob W. Briddon, V. G. Malathi |
| Abstract |
Tomato leaf curl New Delhi virus (ToLCNDV) (Geminiviridae) is an important pathogen that severely affects tomato production. An extensive survey was carried out during 2003-2010 to study the diversity of begomoviruses found in tomato, potato, and cucurbits that showed symptoms of leaf puckering, distortion, curling, vein clearing, and yellow mosaic in various fields in different regions of India. Ten begomovirus isolates were cloned from infected samples and identified as belonging to the species ToLCNDV. A total of 44 % of the samples showed association of betasatellites, with CLCuMuB and LuLDB being the most frequent. The ToLCNDV cloned component DNA A and DNA B were agroinoculated on Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with or without betasatellites, CLCuMuB or LuLDB. The viral genome levels were then monitored by real-time polymerase chain reaction at different time points of disease development. Plants co-inoculated with betasatellites showed enhanced symptom severity in both N. benthamiana and tomato, as well as increases in helper viral DNA A and DNA B levels. The DNA B and betasatellites acted antagonistically to each other, so that the level of DNA B was 16-fold greater in the presence of betasatellites, while accumulation of betasatellites, CLCuMuB and LuLDB, were reduced by 60 % in the presence of DNA B. DNA B-mediated symptoms predominated in CLCuMuB-inoculated plants, whereas betasatellite-mediated leaf abnormalities were prominent in LuLDB-co-inoculated plants. Inoculation with the cloned components will be a good biotechnological tool in resistance breeding program. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 123 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | <1% |
| France | 1 | <1% |
| Unknown | 121 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 31 | 25% |
| Student > Ph. D. Student | 21 | 17% |
| Student > Master | 16 | 13% |
| Researcher | 16 | 13% |
| Other | 5 | 4% |
| Other | 10 | 8% |
| Unknown | 24 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 47 | 38% |
| Unspecified | 31 | 25% |
| Biochemistry, Genetics and Molecular Biology | 8 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
| Nursing and Health Professions | 1 | <1% |
| Other | 5 | 4% |
| Unknown | 29 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2018.
All research outputs
#6,908,521
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,404
of 8,034 outputs
Outputs of similar age
#72,055
of 290,099 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#27
of 56 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 69% of its peers.
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