| Title |
Mechanical loading promotes Lewis lung cancer cell growth through periostin
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, June 2009
|
| DOI | 10.1007/s11626-009-9214-5 |
| Pubmed ID | |
| Authors |
Dedong Ma, Hongxiu Lu, Lisheng Xu, Xia Xu, Wei Xiao |
| Abstract |
Mechanical loading is known to trigger proliferation of tumor cells. Periostin is a new molecule found to increase in many cancers. To determine how mechanical strain modulates tumor growth and its possible mediation by periostin through TGF-beta1, Lewis lung cancer cells were cultured on flexible-bottomed culture plates and cyclically strained using Flexercell Strain Unit. Real-time RT-PCR was used to quantify periostin and TGF-beta1 mRNA levels at 6, 12, 18, and 24 h of loading. In addition, periostin and TGF-beta1 neutralizing antibodies were added to the medium. We showed that the proliferative ability of Lewis cancer cells was significantly increased by cyclical strain. This change can be blocked by 5 microg/ml of periostin neutralizing antibody. Periostin mRNA increased by 1.1-, 3.2-, 4.7-, and 9.2-fold while TGF-beta1 mRNA increased by 5.3-, 10.3-, 7.1-, and 6.5-fold at 6, 12, 18, and 24 h, respectively. Periostin protein in medium increased after cyclical strain. Expression of periostin mRNA in response to mechanical loading was completely blocked by 2.5 microg/ml of TGF-beta1 neutralizing antibody. In addition, overexpression of periostin in Lewis cells can promote cell proliferation. Our results suggest that periostin is a potent positive regulator of tumor growth in response to mechanical loading and is possibly a downstream factor of TGF-beta1. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 28% |
| Researcher | 4 | 22% |
| Other | 3 | 17% |
| Professor > Associate Professor | 2 | 11% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 33% |
| Engineering | 4 | 22% |
| Agricultural and Biological Sciences | 4 | 22% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Unknown | 2 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2013.
All research outputs
#20,178,948
of 22,693,205 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#664
of 788 outputs
Outputs of similar age
#94,724
of 98,523 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#7
of 7 outputs
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So far Altmetric has tracked 788 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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