Title |
Simultaneous mutation of G275A and P276A in the matrix protein of Newcastle disease virus decreases virus replication and budding
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Published in |
Archives of Virology, September 2016
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DOI | 10.1007/s00705-016-3046-x |
Pubmed ID | |
Authors |
Haixu Xu, Zhiqiang Duan, Yu Chen, Jiajia Liu, Xin Cheng, Jingjing Liu, Jie Zhu, Xiaoquan Wang, Xiaowen Liu, Shunlin Hu, Xiufan Liu |
Abstract |
The matrix (M) protein of Newcastle disease virus (NDV) is a highly conserved hydrophobic viral protein. In some paramyxoviruses (measles virus and Sendai virus), the paired glycine (G) near the C terminus of the M protein may form a turn that mediates the specific interaction with the cell membrane. Similar amino acids (glycine-proline [GP], at position 275-276) exist in the M protein of NDV. However, the role of these residues in the replication and pathogenicity of NDV is unknown. In this study, recombinant NDV with the sequence GP/AA or LGP/GGL in the M protein was generated to investigate the role of this conserved sequence. Budding experiments on the mutant viruses revealed that the GP/AA mutation reduced virus budding and virus replication in DF-1 cells; biological characterization revealed attenuated virulence and pathogenicity in chickens, indicating that the GP sequence plays a critical role in the life cycle of the virus. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 25% |
Student > Doctoral Student | 2 | 13% |
Researcher | 2 | 13% |
Lecturer | 1 | 6% |
Student > Bachelor | 1 | 6% |
Other | 4 | 25% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 25% |
Veterinary Science and Veterinary Medicine | 3 | 19% |
Medicine and Dentistry | 2 | 13% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Other | 2 | 13% |
Unknown | 3 | 19% |