Title |
Spheroid culture as a tool for creating 3D complex tissues
|
---|---|
Published in |
Trends in Biotechnology, January 2013
|
DOI | 10.1016/j.tibtech.2012.12.003 |
Pubmed ID | |
Authors |
Eelco Fennema, Nicolas Rivron, Jeroen Rouwkema, Clemens van Blitterswijk, Jan de Boer |
Abstract |
3D cell culture methods confer a high degree of clinical and biological relevance to in vitro models. This is specifically the case with the spheroid culture, where a small aggregate of cells grows free of foreign materials. In spheroid cultures, cells secrete the extracellular matrix (ECM) in which they reside, and they can interact with cells from their original microenvironment. The value of spheroid cultures is increasing quickly due to novel microfabricated platforms amenable to high-throughput screening (HTS) and advances in cell culture. Here, we review new possibilities that combine the strengths of spheroid culture with new microenvironment fabrication methods that allow for the creation of large numbers of highly reproducible, complex tissues. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Netherlands | 2 | 40% |
Nepal | 1 | 20% |
United States | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 80% |
Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | <1% |
Switzerland | 4 | <1% |
United Kingdom | 4 | <1% |
Germany | 2 | <1% |
Canada | 2 | <1% |
Czechia | 1 | <1% |
Israel | 1 | <1% |
New Zealand | 1 | <1% |
Italy | 1 | <1% |
Other | 2 | <1% |
Unknown | 1409 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 320 | 22% |
Student > Master | 235 | 16% |
Researcher | 204 | 14% |
Student > Bachelor | 174 | 12% |
Student > Doctoral Student | 63 | 4% |
Other | 158 | 11% |
Unknown | 280 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 280 | 20% |
Agricultural and Biological Sciences | 240 | 17% |
Engineering | 201 | 14% |
Medicine and Dentistry | 86 | 6% |
Chemistry | 73 | 5% |
Other | 214 | 15% |
Unknown | 340 | 24% |