| Title |
Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates
|
|---|---|
| Published in |
Journal of Parasitic Diseases, September 2014
|
| DOI | 10.1007/s12639-014-0582-8 |
| Pubmed ID | |
| Authors |
K. P. Shyma, S. K. Gupta, J. P. Gupta, Ajit Singh, S. S. Chaudhari, Veer Singh |
| Abstract |
The differences or similarities among different isolates of Trypanosoma evansi through endonuclease profile was identified in the present study. The repetitive nuclear DNA of T. evansi isolated from infected cattle, buffalo and equine blood was initially amplified by PCR using specific primers. A panel of restriction enzymes, EcoRI, Eco91l, HindIII and PstI were for complete digestion of PCR products. Agarose gel electrophoresis of digested product did not show cleavage fragments and only single DNA band of the original size was visible in the ethidium bromide stained agarose gel. This indicated that the 227 bp PCR product from repetitive sequence had no site-specific cleavage sites for the REs used in this study. No heterogeneity in the repetitive nuclear DNA restriction endonuclease profile among the different isolates was recorded. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 40% |
| Unspecified | 1 | 20% |
| Professor > Associate Professor | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Immunology and Microbiology | 1 | 20% |
| Unknown | 2 | 40% |