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Heterologous Expression of Membrane Proteins

Overview of attention for book
Cover of 'Heterologous Expression of Membrane Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Heterologous Expression of Membrane Proteins
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    Chapter 2 Heterologous Expression of Membrane Proteins
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    Chapter 3 Membrane Protein Production in Escherichia coli: Protocols and Rules.
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    Chapter 4 Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach.
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    Chapter 5 Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins.
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    Chapter 6 Membrane Protein Production in Lactococcus lactis for Functional Studies.
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    Chapter 7 Heterologous Expression of Membrane Proteins
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    Chapter 8 Leishmania tarentolae as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6.
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    Chapter 9 Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA.
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    Chapter 10 Heterologous Expression of Membrane Proteins
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    Chapter 11 Heterologous Expression of Membrane Proteins
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    Chapter 12 Heterologous Expression of Membrane Proteins
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    Chapter 13 Heterologous Expression of Membrane Proteins
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    Chapter 14 Heterologous Expression of Membrane Proteins
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    Chapter 15 Membrane Protein Solubilization and Composition of Protein Detergent Complexes.
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    Chapter 16 Detergent-Free Membrane Protein Purification.
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    Chapter 17 Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.
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    Chapter 18 Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization.
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    Chapter 19 Heterologous Expression of Membrane Proteins
Attention for Chapter 16: Detergent-Free Membrane Protein Purification.
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Chapter title
Detergent-Free Membrane Protein Purification.
Chapter number 16
Book title
Heterologous Expression of Membrane Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3637-3_16
Pubmed ID
27485341
Book ISBNs
978-1-4939-3635-9, 978-1-4939-3637-3
Authors

Alice J. Rothnie

Editors

Isabelle Mus-Veteau

Abstract

Membrane proteins are localized within a lipid bilayer; in order to purify them for functional and structural studies the first step must involve solubilizing or extracting the protein from these lipids. To date this has been achieved using detergents which disrupt the bilayer and bind to the protein in the transmembrane region. However finding conditions for optimal extraction, without destabilizing protein structure, is time consuming and expensive. Here we present a recently-developed method using a styrene-maleic acid (SMA) co-polymer instead of detergents. The SMA co-polymer extracts membrane proteins in a small disc of lipid bilayer which can be used for affinity chromatography purification, thus enabling the purification of membrane proteins while maintaining their native lipid bilayer environment.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 63 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 37%
Student > Bachelor 9 14%
Student > Postgraduate 7 11%
Student > Master 4 6%
Researcher 4 6%
Other 1 2%
Unknown 15 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 38%
Agricultural and Biological Sciences 11 17%
Chemistry 7 11%
Pharmacology, Toxicology and Pharmaceutical Science 2 3%
Neuroscience 2 3%
Other 1 2%
Unknown 16 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 January 2019.
All research outputs
#18,471,305
of 22,888,307 outputs
Outputs from Methods in molecular biology
#7,924
of 13,132 outputs
Outputs of similar age
#284,573
of 393,716 outputs
Outputs of similar age from Methods in molecular biology
#845
of 1,471 outputs
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So far Altmetric has tracked 13,132 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.

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