| Title |
Metabolite profiling in Trigonella seeds via UPLC-MS and GC-MS analyzed using multivariate data analyses
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, September 2016
|
| DOI | 10.1007/s00216-016-9910-4 |
| Pubmed ID | |
| Authors |
Mohamed A. Farag, Dalia M. Rasheed, Matthias Kropf, Andreas G. Heiss |
| Abstract |
Trigonella foenum-graecum is a plant of considerable value for its nutritive composition as well as medicinal effects. This study aims to examine Trigonella seeds using a metabolome-based ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) in parallel to gas chromatography-mass spectrometry (GC-MS) coupled with multivariate data analyses. The metabolomic differences of seeds derived from three Trigonella species, i.e., T. caerulea, T. corniculata, and T. foenum-graecum, were assessed. Under specified conditions, we were able to identify 93 metabolites including 5 peptides, 2 phenolic acids, 22 C/O-flavonoid conjugates, 26 saponins, and 9 fatty acids using UPLC-MS. Several novel dipeptides, saponins, and flavonoids were found in Trigonella herein for the first time. Samples were classified via unsupervised principal component analysis (PCA) followed by supervised orthogonal projection to latent structures-discriminant analysis (OPLS-DA). A distinct separation among the investigated Trigonella species was revealed, with T. foenum-graecum samples found most enriched in apigenin-C-glycosides, viz. vicenins 1/3 and 2, compared to the other two species. In contrast to UPLC-MS, GC-MS was less efficient to classify specimens, with differences among specimens mostly attributed to fatty acyl esters. GC-MS analysis of Trigonella seed extracts led to the identification of 91 metabolites belonging mostly to fatty acyl esters, free fatty acids followed by organic acids, sugars, and amino acids. This study presents the first report on primary and secondary metabolite compositional differences among Trigonella seeds via a metabolomics approach and reveals that, among the species examined, the official T. foenum-graecum presents a better source of Trigonella secondary bioactive metabolites. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 74 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 12% |
| Student > Master | 8 | 11% |
| Student > Bachelor | 8 | 11% |
| Researcher | 5 | 7% |
| Student > Doctoral Student | 4 | 5% |
| Other | 12 | 16% |
| Unknown | 28 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 18% |
| Chemistry | 9 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 11% |
| Biochemistry, Genetics and Molecular Biology | 5 | 7% |
| Medicine and Dentistry | 4 | 5% |
| Other | 7 | 9% |
| Unknown | 28 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 October 2016.
All research outputs
#19,945,185
of 25,374,917 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,060
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Outputs of similar age
#246,237
of 335,124 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#69
of 199 outputs
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