| Chapter title |
4D Confocal Imaging of Yeast Organelles
|
|---|---|
| Chapter number | 1 |
| Book title |
The Golgi Complex
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6463-5_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6461-1, 978-1-4939-6463-5
|
| Authors |
Kasey J. Day, Effrosyni Papanikou, Benjamin S. Glick, Day, Kasey J, Papanikou, Effrosyni, Glick, Benjamin S |
| Editors |
William J. Brown |
| Abstract |
Yeast cells are well suited to visualizing organelles by 4D confocal microscopy. Typically, one or more cellular compartments are labeled with a fluorescent protein or dye, and a stack of confocal sections spanning the entire cell volume is captured every few seconds. Under appropriate conditions, organelle dynamics can be observed for many minutes with only limited photobleaching. Images are captured at a relatively low signal-to-noise ratio and are subsequently processed to generate movies that can be analyzed and quantified. Here, we describe methods for acquiring and processing 4D data using conventional scanning confocal microscopy. |
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| Professor | 1 | 5% |
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| Unknown | 5 | 25% |