Title |
The Green Gut: Chlorophyll Degradation in the Gut of Spodoptera littoralis
|
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Published in |
Journal of Chemical Ecology, October 2015
|
DOI | 10.1007/s10886-015-0636-0 |
Pubmed ID | |
Authors |
Amarsanaa Badgaa, Rita Büchler, Natalie Wielsch, Marie Walde, Rainer Heintzmann, Yannik Pauchet, Ales Svatos, Kerstin Ploss, Wilhelm Boland |
Abstract |
Chlorophylls, the most prominent natural pigments, are part of the daily diet of herbivorous insects. The spectrum of ingested and digested chlorophyll metabolites compares well to the pattern of early chlorophyll-degradation products in senescent plants. Intact chlorophyll is rapidly degraded by proteins in the front- and midgut. Unlike plants, insects convert both chlorophyll a and b into the corresponding catabolites. MALDI-TOF/MS imaging allowed monitoring the distribution of the chlorophyll catabolites along the gut of Spodoptera littoralis larvae. The chlorophyll degradation in the fore- and mid-gut is strongly pH dependent, and requires alkaline conditions. Using LC-MS/MS analysis we identified a lipocalin-type protein in the intestinal fluid of S. littoralis homolog to the chlorophyllide a binding protein from Bombyx mori. Widefield and high-resolution autofluorescence microscopy revealed that the brush border membranes are covered with the chlorophyllide binding protein tightly bound via its GPI-anchor to the gut membrane. A function in defense against gut microbes is discussed. |
X Demographics
Geographical breakdown
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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Germany | 1 | 4% |
Unknown | 25 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 19% |
Researcher | 3 | 12% |
Lecturer | 2 | 8% |
Other | 2 | 8% |
Student > Master | 2 | 8% |
Other | 5 | 19% |
Unknown | 7 | 27% |
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Chemistry | 2 | 8% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
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Unspecified | 1 | 4% |
Other | 2 | 8% |
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