| Title |
Continuous detection and genetic diversity of human rotavirus A in sewage in eastern China, 2013–2014
|
|---|---|
| Published in |
Virology Journal, September 2016
|
| DOI | 10.1186/s12985-016-0609-0 |
| Pubmed ID | |
| Authors |
Nan Zhou, Dong Lv, Suting Wang, Xiaojuan Lin, Zhenwang Bi, Haiyan Wang, Pei Wang, Huaning Zhang, Zexin Tao, Peibin Hou, Yanyan Song, Aiqiang Xu |
| Abstract |
Rotavirus is the leading viral agent for pediatric gastroenteritis. However, the case-based surveillance for rotavirus is limited in China, and its circulation in the environment is not well investigated. From 2013 to 2014, rotavirus was detected in raw sewage samples of Jinan and Linyi by quantitative PCR (qPCR) and conventional reverse transcription PCR (RT-PCR). After sequenced and genotyped, sequences analysis was conducted. A total of 46 sewage samples were collected monthly for the detection of rotavirus, and rotavirus was positive in 43 samples (93.5 %, 43/46). By quantitative assessment, the concentrations of rotavirus in raw sewage ranged from 4.1 × 10(3) to 1.3 × 10(6) genome copies (GC)/L in Jinan, and from 1.5 × 10(3) to 3.0 × 10(5) GC/L in Linyi. A total of 318 sequences of 5 G-genotypes and 318 sequences of 5 P-genotypes were obtained. G9 (91.8 %, 292/318) and P[8] (56.0 %, 178/318) were the most common G- and P-genotype, respectively. Multiple transmission lineages were recognized in these genotypes. Interestingly, an intragenic recombination event between two G9 lineages was observed. This study provided the first report of comprehensive environmental surveillance for rotavirus in China. The results suggest that the concentration of rotavirus in raw sewage was high, and multiple rotavirus transmission lineages continuously co-circulated in Shandong. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 16% |
| Student > Doctoral Student | 3 | 9% |
| Student > Ph. D. Student | 3 | 9% |
| Student > Master | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Other | 4 | 13% |
| Unknown | 12 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 9% |
| Environmental Science | 2 | 6% |
| Immunology and Microbiology | 2 | 6% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 4 | 13% |
| Unknown | 19 | 59% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2016.
All research outputs
#15,384,302
of 22,888,307 outputs
Outputs from Virology Journal
#1,962
of 3,051 outputs
Outputs of similar age
#203,481
of 322,146 outputs
Outputs of similar age from Virology Journal
#27
of 45 outputs
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