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Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube

Overview of attention for article published in Journal of Microbiological Methods, February 2003
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  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

patent
4 patents
facebook
1 Facebook page

Citations

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34 Dimensions

Readers on

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41 Mendeley
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Title
Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
Published in
Journal of Microbiological Methods, February 2003
DOI 10.1016/s0167-7012(02)00163-x
Pubmed ID
Authors

Edson Bertolini, Ramón Penyalver, Amparo Garcı́a, Antonio Olmos, José M Quesada, Mariano Cambra, Marı́a M López

Abstract

A nested-polymerase chain reaction (PCR) has been set up to be performed in a single closed tube for the detection of Pseudomonas savastanoi pv. savastanoi. Nested-PCR coupled with dot-blot hybridization was able to detect up to one cell of the target per ml of olive extract, showing the greatest sensitivity compared with all previously reported detection assays. Validation of the developed procedure for diagnosis and epidemiological purposes was achieved by testing ca. 240 asymptomatic plant samples from olive trees. When performing the other previously reported techniques (bacterial isolation and single PCR), P. savastanoi was detected in 50 of the analyzed samples, while with the new developed nested-PCR assay, the bacterium was detected in 82 samples.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Greece 1 2%
France 1 2%
Brazil 1 2%
Unknown 37 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 32%
Student > Ph. D. Student 7 17%
Student > Doctoral Student 3 7%
Professor 3 7%
Student > Master 3 7%
Other 4 10%
Unknown 8 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 59%
Biochemistry, Genetics and Molecular Biology 2 5%
Medicine and Dentistry 2 5%
Computer Science 1 2%
Arts and Humanities 1 2%
Other 2 5%
Unknown 9 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2013.
All research outputs
#8,262,107
of 25,374,647 outputs
Outputs from Journal of Microbiological Methods
#674
of 2,350 outputs
Outputs of similar age
#33,590
of 140,949 outputs
Outputs of similar age from Journal of Microbiological Methods
#7
of 14 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 2,350 research outputs from this source. They receive a mean Attention Score of 3.7. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 140,949 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.