| Title |
Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome
|
|---|---|
| Published in |
Metabolomics, September 2016
|
| DOI | 10.1007/s11306-016-1109-3 |
| Pubmed ID | |
| Authors |
Dorothea Lesche, Roland Geyer, Daniel Lienhard, Christos T. Nakas, Gaëlle Diserens, Peter Vermathen, Alexander B. Leichtle |
| Abstract |
Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses. Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements. Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols. Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies. |
X Demographics
The data shown below were collected from the profiles of 15 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 27% |
| Spain | 2 | 13% |
| India | 1 | 7% |
| Unknown | 8 | 53% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 60% |
| Scientists | 4 | 27% |
| Science communicators (journalists, bloggers, editors) | 2 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 1% |
| Germany | 1 | 1% |
| South Africa | 1 | 1% |
| Unknown | 86 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 15 | 17% |
| Student > Bachelor | 15 | 17% |
| Researcher | 14 | 16% |
| Student > Ph. D. Student | 6 | 7% |
| Student > Doctoral Student | 5 | 6% |
| Other | 17 | 19% |
| Unknown | 17 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 19% |
| Agricultural and Biological Sciences | 15 | 17% |
| Chemistry | 12 | 13% |
| Medicine and Dentistry | 5 | 6% |
| Immunology and Microbiology | 4 | 4% |
| Other | 15 | 17% |
| Unknown | 21 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 April 2017.
All research outputs
#3,623,366
of 23,301,510 outputs
Outputs from Metabolomics
#174
of 1,309 outputs
Outputs of similar age
#59,642
of 322,508 outputs
Outputs of similar age from Metabolomics
#6
of 38 outputs
Altmetric has tracked 23,301,510 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,309 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 322,508 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.