| Title |
Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
|
|---|---|
| Published in |
Microbial Cell Factories, September 2016
|
| DOI | 10.1186/s12934-016-0555-y |
| Pubmed ID | |
| Authors |
José Antonio Curiel, Zoel Salvadó, Jordi Tronchoni, Pilar Morales, Alda Joao Rodrigues, Manuel Quirós, Ramón Gonzalez |
| Abstract |
Aerobic fermentation of grape must, leading to respiro-fermentative metabolism of sugars, has been proposed as way of reducing alcohol content in wines. Two factors limit the usefulness of Saccharomyces cerevisiae for this application, the Crabtree effect, and excess volatile acidity under aerobic conditions. This work aimed to explore the impact on ethanol acetate production of different S. cerevisiae strains deleted for genes previously related with the Crabtree phenotype. Recombinant strains were constructed on a wine industrial genetic background, FX10. All yeast strains, including FX10, showed respiro-fermentative metabolism in natural grape must under aerobic conditions, as well as a concomitant reduction in ethanol yield. This indicates that the Crabtree effect is not a major constrain for reaching relevant respiration levels in grape must. Indeed, only minor differences in ethanol yield were observed between the original and some of the recombinant strains. In contrast, some yeast strains showed a relevant reduction of acetic acid production. This was identified as a positive feature for the feasibility of alcohol level reduction by respiration. Reduced acetic acid production was confirmed by a thorough analysis of these and some additional deletion strains (involving genes HXK2, PYK1, REG1, PDE2 and PDC1). Some recombinant yeasts showed altered production of glycerol and pyruvate derived metabolites. REG1 and PDC1 deletion strains showed a strong reduction of acetic acid yield in aerobic fermentations. Since REG1 defective strains may be obtained by non-GMO approaches, these gene modifications show good promise to help reducing ethanol content in wines. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 2% |
| France | 1 | 2% |
| Unknown | 42 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 25% |
| Student > Bachelor | 7 | 16% |
| Student > Ph. D. Student | 6 | 14% |
| Student > Doctoral Student | 4 | 9% |
| Student > Master | 4 | 9% |
| Other | 4 | 9% |
| Unknown | 8 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 34% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Engineering | 5 | 11% |
| Chemistry | 5 | 11% |
| Physics and Astronomy | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 8 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 July 2022.
All research outputs
#4,192,766
of 22,888,307 outputs
Outputs from Microbial Cell Factories
#227
of 1,604 outputs
Outputs of similar age
#69,310
of 321,166 outputs
Outputs of similar age from Microbial Cell Factories
#2
of 38 outputs
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So far Altmetric has tracked 1,604 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 84% of its peers.
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