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Arginase Treatment Prevents the Recovery of Canine Lymphoma and Osteosarcoma Cells Resistant to the Toxic Effects of Prolonged Arginine Deprivation

Overview of attention for article published in PLOS ONE, January 2013
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  • Good Attention Score compared to outputs of the same age (69th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

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Title
Arginase Treatment Prevents the Recovery of Canine Lymphoma and Osteosarcoma Cells Resistant to the Toxic Effects of Prolonged Arginine Deprivation
Published in
PLOS ONE, January 2013
DOI 10.1371/journal.pone.0054464
Pubmed ID
Authors

James W. Wells, Christopher H. Evans, Milcah C. Scott, Barbara C. Rütgen, Timothy D. O'Brien, Jaime F. Modiano, Goran Cvetkovic, Slobodan Tepic

Abstract

Rapidly growing tumor cells require a nutrient-rich environment in order to thrive, therefore, restricting access to certain key amino acids, such as arginine, often results in the death of malignant cells, which frequently display defective cell cycle check-point control. Healthy cells, by contrast, become quiescent and remain viable under arginine restriction, displaying full recovery upon return to arginine-rich conditions. The use of arginase therapy to restrict available arginine for selectively targeting malignant cells is currently under investigation in human clinical trials. However, the suitability of this approach for veterinary uses is unexplored. As a prelude to in vivo studies in canine malignancies, we examined the in vitro effects of arginine-deprivation on canine lymphoid and osteosarcoma cell lines. Two lymphoid and 2 osteosarcoma cell lines were unable to recover following 6 days of arginine deprivation, but all remaining cell lines displayed full recovery upon return to arginine-rich culture conditions. These remaining cell lines all proved susceptible to cell death following the addition of arginase to the cultures. The lymphoid lines were particularly sensitive to arginase, becoming unrecoverable after just 3 days of treatment. Two of the osteosarcoma lines were also susceptible over this time-frame; however the other 3 lines required 6-8 days of arginase treatment to prevent recovery. In contrast, adult progenitor cells from the bone marrow of a healthy dog were able to recover fully following 9 days of culture in arginase. Over 3 days in culture, arginase was more effective than asparaginase in inducing the death of lymphoid lines. These results strongly suggest that short-term arginase treatment warrants further investigation as a therapy for lymphoid malignancies and osteosarcomas in dogs.

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X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 5%
Unknown 57 95%

Demographic breakdown

Readers by professional status Count As %
Other 10 17%
Student > Ph. D. Student 9 15%
Student > Postgraduate 8 13%
Researcher 7 12%
Student > Bachelor 5 8%
Other 15 25%
Unknown 6 10%
Readers by discipline Count As %
Medicine and Dentistry 14 23%
Agricultural and Biological Sciences 13 22%
Veterinary Science and Veterinary Medicine 11 18%
Biochemistry, Genetics and Molecular Biology 5 8%
Unspecified 3 5%
Other 7 12%
Unknown 7 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 February 2013.
All research outputs
#8,579,659
of 25,501,527 outputs
Outputs from PLOS ONE
#114,702
of 222,333 outputs
Outputs of similar age
#88,632
of 288,588 outputs
Outputs of similar age from PLOS ONE
#2,110
of 5,033 outputs
Altmetric has tracked 25,501,527 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 222,333 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.8. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 288,588 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 5,033 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.