Title |
LTP Requires a Unique Postsynaptic SNARE Fusion Machinery
|
---|---|
Published in |
Neuron, February 2013
|
DOI | 10.1016/j.neuron.2012.11.029 |
Pubmed ID | |
Authors |
Sandra Jurado, Debanjan Goswami, Yingsha Zhang, Alfredo J. Miñano Molina, Thomas C. Südhof, Robert C. Malenka |
Abstract |
Membrane fusion during exocytosis is mediated by assemblies of SNARE (soluble NSF-attachment protein receptor) and SM (Sec1/Munc18-like) proteins. The SNARE/SM proteins involved in vesicle fusion during neurotransmitter release are well understood, whereas little is known about the protein machinery that mediates activity-dependent AMPA receptor (AMPAR) exocytosis during long-term potentiation (LTP). Using direct measurements of LTP in acute hippocampal slices and an in vitro LTP model of stimulated AMPAR exocytosis, we demonstrate that the Q-SNARE proteins syntaxin-3 and SNAP-47 are required for regulated AMPAR exocytosis during LTP but not for constitutive basal AMPAR exocytosis. In contrast, the R-SNARE protein synaptobrevin-2/VAMP2 contributes to both regulated and constitutive AMPAR exocytosis. Both the central complexin-binding and the N-terminal Munc18-binding sites of syntaxin-3 are essential for its postsynaptic role in LTP. Thus, postsynaptic exocytosis of AMPARs during LTP is mediated by a unique fusion machinery that is distinct from that used during presynaptic neurotransmitter release. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 17% |
India | 1 | 17% |
United States | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 5 | 83% |
Scientists | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | 2% |
Japan | 3 | <1% |
Switzerland | 1 | <1% |
Chile | 1 | <1% |
France | 1 | <1% |
Netherlands | 1 | <1% |
Austria | 1 | <1% |
Finland | 1 | <1% |
India | 1 | <1% |
Other | 5 | 1% |
Unknown | 312 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 79 | 24% |
Researcher | 66 | 20% |
Student > Bachelor | 36 | 11% |
Student > Master | 26 | 8% |
Professor | 18 | 5% |
Other | 56 | 17% |
Unknown | 53 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 143 | 43% |
Neuroscience | 77 | 23% |
Medicine and Dentistry | 18 | 5% |
Biochemistry, Genetics and Molecular Biology | 17 | 5% |
Nursing and Health Professions | 3 | <1% |
Other | 18 | 5% |
Unknown | 58 | 17% |