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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Ordering a Dynamic Protein Via a Small-Molecule Stabilizer
|
|---|---|
| Published in |
Journal of the American Chemical Society, February 2013
|
| DOI | 10.1021/ja3122334 |
| Pubmed ID | |
| Authors |
Ningkun Wang, Chinmay Y. Majmudar, William C. Pomerantz, Jessica K. Gagnon, Jack D. Sadowsky, Jennifer L. Meagher, Taylor K. Johnson, Jeanne A. Stuckey, Charles L. Brooks, James A. Wells, Anna K. Mapp |
| Abstract |
Like many coactivators, the GACKIX domain of the master coactivator CBP/p300 recognizes transcriptional activators of diverse sequence composition via dynamic binding surfaces. The conformational dynamics of GACKIX that underlie its function also render it especially challenging for structural characterization. We have found that the ligand discovery strategy of Tethering is an effective method for identifying small-molecule fragments that stabilize the GACKIX domain, enabling for the first time the crystallographic characterization of this important motif. The 2.0 Å resolution structure of GACKIX complexed to a small molecule was further analyzed by molecular dynamics simulations, which revealed the importance of specific side-chain motions that remodel the activator binding site in order to accommodate binding partners of distinct sequence and size. More broadly, these results suggest that Tethering can be a powerful strategy for identifying small-molecule stabilizers of conformationally malleable proteins, thus facilitating their structural characterization and accelerating the discovery of small-molecule modulators. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Germany | 1 | 20% |
| United Kingdom | 1 | 20% |
| Unknown | 2 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
| Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Italy | 1 | <1% |
| France | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 108 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 40 | 36% |
| Researcher | 21 | 19% |
| Student > Doctoral Student | 7 | 6% |
| Professor | 6 | 5% |
| Other | 6 | 5% |
| Other | 21 | 19% |
| Unknown | 11 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 53 | 47% |
| Agricultural and Biological Sciences | 18 | 16% |
| Biochemistry, Genetics and Molecular Biology | 16 | 14% |
| Unspecified | 3 | 3% |
| Computer Science | 2 | 2% |
| Other | 5 | 4% |
| Unknown | 15 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2013.
All research outputs
#7,787,627
of 24,189,858 outputs
Outputs from Journal of the American Chemical Society
#29,688
of 64,261 outputs
Outputs of similar age
#63,601
of 196,284 outputs
Outputs of similar age from Journal of the American Chemical Society
#293
of 567 outputs
Altmetric has tracked 24,189,858 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 64,261 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one has gotten more attention than average, scoring higher than 53% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 196,284 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 567 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.