| Title |
The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more
|
|---|---|
| Published in |
Journal of Structural and Functional Genomics, November 2011
|
| DOI | 10.1007/s10969-011-9118-y |
| Pubmed ID | |
| Authors |
Yvonne Thielmann, Juergen Koepke, Hartmut Michel |
| Abstract |
Structure determination of membrane proteins and membrane protein complexes is still a very challenging field. To facilitate the work on membrane proteins the Core Centre follows a strategy that comprises four labs of protein analytics and crystal handling, covering mass spectrometry, calorimetry, crystallization and X-ray diffraction. This general workflow is presented and a capacity of 20% of the operating time of all systems is provided to the European structural biology community within the ESFRI Instruct program. A description of the crystallization service offered at the Core Centre is given with detailed information on screening strategy, screens used and changes to adapt high throughput for membrane proteins. Our aim is to constantly develop the Core Centre towards the usage of more efficient methods. This strategy might also include the ability to automate all steps from crystallization trials to crystal screening; here we look ahead how this aim might be realized at the Core Centre. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 20% |
| Other | 2 | 10% |
| Student > Master | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Other | 3 | 15% |
| Unknown | 6 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 15% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Earth and Planetary Sciences | 2 | 10% |
| Computer Science | 2 | 10% |
| Unspecified | 1 | 5% |
| Other | 4 | 20% |
| Unknown | 6 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2013.
All research outputs
#18,329,207
of 22,696,971 outputs
Outputs from Journal of Structural and Functional Genomics
#87
of 107 outputs
Outputs of similar age
#195,168
of 238,929 outputs
Outputs of similar age from Journal of Structural and Functional Genomics
#2
of 2 outputs
Altmetric has tracked 22,696,971 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 107 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,929 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.