Title |
Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL
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Published in |
Virchows Archiv, September 2016
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DOI | 10.1007/s00428-016-2025-7 |
Pubmed ID | |
Authors |
Zandra C Deans, Jose Luis Costa, Ian Cree, Els Dequeker, Anders Edsjö, Shirley Henderson, Michael Hummel, Marjolijn JL Ligtenberg, Marco Loddo, Jose Carlos Machado, Antonio Marchetti, Katherine Marquis, Joanne Mason, Nicola Normanno, Etienne Rouleau, Ed Schuuring, Keeda-Marie Snelson, Erik Thunnissen, Bastiaan Tops, Gareth Williams, Han van Krieken, Jacqueline A Hall, On behalf of IQN Path ASBL |
Abstract |
The clinical demand for mutation detection within multiple genes from a single tumour sample requires molecular diagnostic laboratories to develop rapid, high-throughput, highly sensitive, accurate and parallel testing within tight budget constraints. To meet this demand, many laboratories employ next-generation sequencing (NGS) based on small amplicons. Building on existing publications and general guidance for the clinical use of NGS and learnings from germline testing, the following guidelines establish consensus standards for somatic diagnostic testing, specifically for identifying and reporting mutations in solid tumours. These guidelines cover the testing strategy, implementation of testing within clinical service, sample requirements, data analysis and reporting of results. In conjunction with appropriate staff training and international standards for laboratory testing, these consensus standards for the use of NGS in molecular pathology of solid tumours will assist laboratories in implementing NGS in clinical services. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | <1% |
Italy | 1 | <1% |
Norway | 1 | <1% |
Unknown | 147 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 25 | 17% |
Student > Ph. D. Student | 23 | 15% |
Researcher | 22 | 15% |
Student > Bachelor | 17 | 11% |
Student > Master | 12 | 8% |
Other | 21 | 14% |
Unknown | 30 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 40 | 27% |
Biochemistry, Genetics and Molecular Biology | 37 | 25% |
Agricultural and Biological Sciences | 16 | 11% |
Nursing and Health Professions | 7 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
Other | 13 | 9% |
Unknown | 34 | 23% |