| Title |
19F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy
|
|---|---|
| Published in |
Glycoconjugate Journal, August 2016
|
| DOI | 10.1007/s10719-016-9723-x |
| Pubmed ID | |
| Authors |
Marcelo A. Lima, Renan P. Cavalheiro, Gustavo M.Viana, Maria C.Z. Meneghetti, Timothy R. Rudd, Mark A. Skidmore, Andrew K. Powell, Edwin A. Yates |
| Abstract |
Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, often, this is not feasible. The cell surface and extracellular matrix polysaccharides, glycosaminoglycans (GAGs) lack groups that can be detected selectively in the biological milieu. The introduction of (19)F labels into GAG polysaccharides is explored and the interaction of a labelled GAG with the heparin-binding protein, antithrombin, employing (19)F NMR spectroscopy is followed. Furthermore, the ability of (19)F labelled GAGs to be imaged using CARS microscopy is demonstrated. (19)F labelled GAGs enable both (19)F NMR protein-GAG binding studies in solution at the molecular level and non-linear microscopy at a microscopic scale to be conducted on the same material, essentially free of background signals. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 18% |
| Other | 2 | 12% |
| Student > Ph. D. Student | 2 | 12% |
| Lecturer | 1 | 6% |
| Professor | 1 | 6% |
| Other | 3 | 18% |
| Unknown | 5 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 35% |
| Agricultural and Biological Sciences | 2 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Chemistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 6 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2016.
All research outputs
#22,759,802
of 25,374,917 outputs
Outputs from Glycoconjugate Journal
#870
of 929 outputs
Outputs of similar age
#316,041
of 356,506 outputs
Outputs of similar age from Glycoconjugate Journal
#17
of 20 outputs
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So far Altmetric has tracked 929 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.